Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum , Causing Fusarium Head Blight in Wheat

• Published in: Toxins Vol. 11; no. 5; p. 272
• Main Authors: Xu, Chao, Li, Meixia, Zhou, Zehua, Li, Jiaosheng, Chen, Dongming, Duan, Yabing, Zhou, Mingguo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.05.2019; MDPI
• Subjects: Acetyl Coenzyme A - metabolism; Acids; Biosynthesis; Blight; Cereal crops; Citric acid; Citric Acid - metabolism; Contamination; Dehydrogenase; Dehydrogenases; Deoxynivalenol; Enzymes; Flutolanil; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungicides; Fungicides, Industrial - pharmacology; Fusarium; Fusarium - drug effects; Fusarium - metabolism; Fusarium asiaticum; Fusarium graminearum; Fusarium head blight; Gene expression; Gene Expression - drug effects; Germination; Glycolysis; Glycolysis - drug effects; Inhibitors; Iron-sulfur proteins; Isocitrate dehydrogenase; Isocitrate Dehydrogenase - metabolism; Kinases; Mitochondria; Mitochondria - drug effects; Mitochondria - enzymology; Mycelia; Mycelium - drug effects; Mycelium - growth & development; Mycotoxins; NADH; Nicotinamide adenine dinucleotide; Pathogens; Pesticides; Plant diseases; Plant Diseases - microbiology; Pyruvic acid; Pyruvic Acid - metabolism; Spore germination; Spores, Fungal - drug effects; Succinate dehydrogenase; Succinate Dehydrogenase - antagonists & inhibitors; Succinate Dehydrogenase - metabolism; succinate dehydrogenase inhibitor; toxisomes; Tri5 gene; Tricarboxylic acid cycle; Trichothecenes; Trichothecenes - metabolism; Triticum - microbiology; Wheat; China; deoxynivalenol; tricarboxylic acid cycle; Fusarium asiaticum; toxisomes; succinate dehydrogenase inhibitor; glycolysis
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins11050272

Deoxynivalenol (DON) is a class of mycotoxin produced in cereal crops infected with species complex (FGSC). In China, FGSC mainly includes and . DON belongs to the trichothecenes and poses a serious threat to the safety and health of humans and animals. Succinate dehydrogenase inhibitors (SDHIs) are a class of fungicides that act on succinate dehydrogenase and inhibit the respiration of pathogenic fungi. In this study, the fungicidal activities of five SDHIs, including fluopyram, flutolanil, boscalid, benzovindiflupyr, and fluxapyroxad, against FGSC were determined based on mycelial growth and spore germination inhibition methods. The five SDHIs exhibited better inhibitory activities in spore germination than mycelial growth. Fluopyram exhibited a higher inhibitory effect in mycelial growth and spore germination in comparison to the other four SDHIs. In addition, the biological characteristics of as affected by the five SDHIs were determined. We found that these five SDHIs decreased DON, pyruvic acid and acetyl-CoA production, isocitrate dehydrogenase mitochondrial (ICDHm) and SDH activities, and NADH and ATP content of but increased the citric acid content. In addition, gene expression was inhibited, and the formation of toxisomes was disrupted by the five SDHIs, further confirming that SDHIs can decrease DON biosynthesis of . Thus, we concluded that SDHIs may decrease DON biosynthesis of by inhibiting glycolysis and the tricarboxylic acid (TCA) cycle. Overall, the findings from the study will provide important references for managing Fusarium head blight (FHB) caused by FGSC and reducing DON contamination in -infected wheat grains.