Methylation of desmethyl analogue of Y nucleosides. Wyosine from guanosine

Wyoslne la. one of the fluorescent hypermodified Y nucleosides found In tRNAsPhe, was synthesized chemically from its biogenetic precursor guanosine 2. The route involved transformation of 2. into the tricyclic structure 3a and subsequent methylation at N-4. The major products of various methylation...

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Bibliographic Details
Published inNucleic acids research Vol. 11; no. 15; pp. 5243 - 6255
Main Authors Golankiewicz, Boźenna, Folkman, Wojciech
Format Journal Article
LanguageEnglish
Published England Oxford University Press 11.08.1983
Subjects
Guanosine - analogs & derivatives
Guanosine - chemical synthesis
Indicators and Reagents
Isomerism
Magnetic Resonance Spectroscopy
Methods
Methylation
RNA - analysis
Spectrophotometry, Ultraviolet
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Summary:Wyoslne la. one of the fluorescent hypermodified Y nucleosides found In tRNAsPhe, was synthesized chemically from its biogenetic precursor guanosine 2. The route involved transformation of 2. into the tricyclic structure 3a and subsequent methylation at N-4. The major products of various methylation procedures were isomers of wyosine, methylated at H-5 (5b) or at H-l (4). Mesoionic compound 4 is a new analogue of 7-methylguanosine 5, modified nucl-eoside occuring in the unique positions in transfer, messenger and ribosomal HNAs. The chromatographio and spectral ohaxacteristios of wyosine and its isomers is given.
Bibliography:ark:/67375/HXZ-JWLBSLCK-R
istex:11935E167AFA0F00AA35D8B92E0451CF434FC041
ArticleID:11.15.5243
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/11.15.5243