Multiomic Approaches Reveal Hormonal Modulation and Nitrogen Uptake and Assimilation in the Initial Growth of Maize Inoculated with Herbaspirillum seropedicae

• Published in: Plants (Basel) Vol. 12; no. 1; p. 48
• Main Authors: Irineu, Luiz Eduardo Souza da Silva, Soares, Cleiton de Paula, Soares, Tatiane Sanches, Almeida, Felipe Astolpho de, Almeida-Silva, Fabrício, Gazara, Rajesh Kumar, Meneses, Carlos Henrique Salvino Gadelha, Canellas, Luciano Pasqualoto, Silveira, Vanildo, Venancio, Thiago Motta, Olivares, Fabio Lopes
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.12.2022; MDPI
• Subjects: Agricultural production; Agriculture; Amino acids; Assimilation; Bacteria; biofertilizer; Biological assimilation; Biomass; biostimulation; Carbon; Corn; Data analysis; diazotrophs; Endophytes; endophytic bacteria; Fertilizers; Genes; Grasses; Herbaspirillum seropedicae; Herbaspirillum spp; Inoculation; Kinases; Metabolism; Microorganisms; Modulation; Nitrogen; Nitrogen metabolism; Nutrient uptake; Optimization; Phenotypes; Phytohormones; Plant growth; plant-growth promotion; Polyamines; Proteasomes; Proteins; Proteomics; Seedlings; Transcriptomics; Tricarboxylic acid cycle; Ubiquitin; Brazil; biofertilizer; diazotrophs; biostimulation; Herbaspirillum spp; endophytic bacteria; plant-growth promotion
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12010048

is an endophytic bacterium that can fix nitrogen and synthesize phytohormones, which can lead to a plant growth-promoting effect when used as a microbial inoculant. Studies focused on mechanisms of action are crucial for a better understanding of the bacteria-plant interaction and optimization of plant growth-promoting response. This work aims to understand the underlined mechanisms responsible for the early stimulatory growth effects of inoculation in maize. To perform these studies, we combined transcriptomic and proteomic approaches with physiological analysis. The results obtained eight days after inoculation (d.a.i) showed increased root biomass (233 and 253%) and shoot biomass (249 and 264%), respectively, for the fresh and dry mass of maize-inoculated seedlings and increased green content and development. Omics data analysis, before a positive biostimulation phenotype (5 d.a.i.) revealed that inoculation increases N-uptake and N-assimilation machinery through differentially expressed nitrate transporters and amino acid pathways, as well carbon/nitrogen metabolism integration by the tricarboxylic acid cycle and the polyamine pathway. Additionally, phytohormone levels of root and shoot tissues increased in bacterium-inoculated-maize plants, leading to feedback regulation by the ubiquitin-proteasome system. The early biostimulatory effect of partially results from hormonal modulation coupled with efficient nutrient uptake-assimilation and a boost in primary anabolic metabolism of carbon-nitrogen integrative pathways.