The Inhibitory Effect of Pseudomonas stutzeri YM6 on Aspergillus flavus Growth and Aflatoxins Production by the Production of Volatile Dimethyl Trisulfide

• Published in: Toxins Vol. 14; no. 11; p. 788
• Main Authors: Gong, An-Dong, Lei, Yin-Yu, He, Wei-Jie, Liao, Yu-Cai, Ma, Ling, Zhang, Tian-Tian, Zhang, Jing-Bo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.11.2022; MDPI
• Subjects: Aflatoxins; Aflatoxins - analysis; Aspergillus flavus; Aspergillus flavus - metabolism; Biological control; Carcinogens; Conidia; Corn; Crop production; Cytology; Dimethyl trisulfide; Food security; Fungi; Fungicides; Gas chromatography; Germination; Humans; Infections; Liver cancer; Mass spectrometry; Mass spectroscopy; Metabolites; Mycelia; Nuts; Peanuts; Pesticides; Phylogeny; Physical characteristics; post-harvest control; Pseudomonas; Pseudomonas stutzeri; Relative abundance; Scanning electron microscopy; Strain analysis; Volatile compounds; Volatiles; United States > US; China; aflatoxins; Aspergillus flavus; post-harvest control; Pseudomonas stutzeri; dimethyl trisulfide
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins14110788

and the produced aflatoxins cause great hazards to food security and human health across all countries. The control of and aflatoxins in grains during storage is of great significance to humans. In the current study, bacteria strain YM6 isolated from sea sediment was demonstrated effective in controlling by the production of anti-fungal volatiles. According to morphological characteristics and phylogenetic analysis, strain YM6 was identified as YM6 can produce abundant volatile compounds which could inhibit mycelial growth and conidial germination of . Moreover, it greatly prevented fungal infection and aflatoxin production on maize and peanuts during storage. The inhibition rate was 100%. Scanning electron microscopy further supported that the volatiles could destroy the cell structure of and prevent conidia germination on the grain surface. Gas chromatography/mass spectrometry revealed that dimethyl trisulfide (DMTS) with a relative abundance of 13% is the most abundant fraction in the volatiles from strain YM6. The minimal inhibitory concentration of DMTS to conidia is 200 µL/L (compound volume/airspace volume). Thus, we concluded that YM6 and the produced DMTS showed great inhibition to , which could be considered as effective biocontrol agents in further application.