Paenibacillus marinisediminis sp. nov., a bacterium isolated from marine sediment

• Published in: The journal of microbiology Vol. 51; no. 3; pp. 312 - 317
• Main Authors: Lee, H.W., Kyung Hee University, Seoul, Republic of Korea, Roh, S.W., Kyung Hee University, Seoul, Republic of Korea, Yim, K.J., Korea Basic Science Institute, Jeju, Republic of Korea, Shin, N.R., Korea Basic Science Institute, Jeju, Republic of Korea, Lee, J.N., Kyung Hee University, Seoul, Republic of Korea, Whon, T.W., Kyung Hee University, Seoul, Republic of Korea, Kim, J.Y., Kyung Hee University, Seoul, Republic of Korea, Hyun, D.W., Korea Basic Science Institute, Jeju, Republic of Korea, Kim, D.K., Korea Basic Science Institute, Jeju, Republic of Korea, Bae, J.W., Korea Basic Science Institute, Jeju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.06.2013; The Microbiological Society of Korea; Springer Nature B.V; 한국미생물학회
• Subjects: BACILLUS; Bacteria; bacterial taxonomy,marine sediment,Paenibacillus marinisediminis sp. nov; Base Composition - genetics; Biomedical and Life Sciences; coasts; Deoxyribonucleic acid; Diaminopimelic Acid - metabolism; DNA; fatty acids; Geologic Sediments - microbiology; Growth conditions; isoprenoids; Life Sciences; Lipids; Marine sediments; Microbial Systematics and Evolutionary Microbiology; Microbiology; new species; nucleic acid hybridization; nucleotide sequences; Paenibacillus; Paenibacillus - classification; Paenibacillus - genetics; Paenibacillus - metabolism; peptidoglycans; phosphatidylethanolamines; Phosphatidylethanolamines - metabolism; Phosphatidylglycerols - metabolism; Phospholipids - metabolism; Phylogenetics; Phylogeny; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sediments; Sodium chloride; spermidine; Spermidine - metabolism; Taxonomy; 생물학; South Korea; United States > US; INW, Korea, Rep; marine sediment; sp. nov; bacterial taxonomy
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-013-3198-2

A Gram-negative, nonmotile, endospore-forming, rod-shaped bacterial strain LHW35T, which belonged to the genus Paenibacillus, was isolated from marine sediment collected from the south coast of the Republic of Korea. A phylogenetic analysis of 16S rRNA gene sequences indicated that strain LHW35T was most closely related to Paenibacillus taiwanensis G-soil-2-3T (97.2% similarity). The optimal growth conditions for strain LHW35T were 37°C, pH 6.0, and 0% (w/v) NaCl. The main isoprenoid quinone was menaquinone-7 (MK-7) and the major polyamine was spermidine. The diamino acid present in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major fatty acids were anteiso-C15:0 and C16:0. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified amino-hospholipids, unidentified phospholipids, and unidentified polar lipids. A DNA-DNA hybridization experiment using the type strain of P. taiwanensis indicated 40% relatedness. The DNA G+C content was 45.0 mol%. Based on these phylogenetic, genomic, and phenotypic analyses, strain LHW35T should be classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus marinisediminis sp. nov. is proposed. The type strain is LHW35T (