Host Resistance to Uromyces appendiculatus in Common Bean Genotypes

• Published in: Plants (Basel) Vol. 11; no. 5; p. 628
• Main Authors: Omara, Reda Ibrahim, Kamel, Said Mohamed, El-Ganainy, Sherif Mohamed, Arafa, Ramadan Ahmed, Mostafa, Yasser Sabry, Alamri, Saad Abdulrahman, Alrumman, Sulaiman A, Hashem, Mohamed, Elsharkawy, Mohsen Mohamed
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.02.2022; MDPI
• Subjects: Ammonia; antioxidant enzymes; Antioxidants; Apoptosis; Beans; Catalase; common bean; Crop yield; disease parameters; Disease resistance; Electrolyte leakage; Electrolytes; Enzymes; Genotypes; Growing season; Humidity; Hydrogen peroxide; Infections; molecular markers; Oxidation resistance; Oxidative stress; Pathogens; Peroxidase; Phaseolus vulgaris; Phenolic compounds; Phenols; Phenylalanine; Physiology; Polyphenol oxidase; reactive oxygen species; rust; Rust fungi; Seeds; Transcription; Uromyces appendiculatus; rust; common bean; disease parameters; reactive oxygen species; antioxidant enzymes; molecular markers; yield components
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11050628

Rust, induced by the fungus , is one of the most serious bean diseases. The involved mechanisms in rust resistance were evaluated in 10 common bean genotypes during the 2019/2020 and 2020/2021 growing seasons. The disease parameters such as final rust severity (FRS%), area under the disease progress curve (AUDPC) and disease increase rate (r-value) were lower in the resistant genotypes than in highly susceptible genotypes. Biochemical compounds such as total phenols and the activity of antioxidant enzymes such as catalase, peroxidase and polyphenol oxidase were increased in the resistant genotypes compared to susceptible genotypes. In the resistance genotypes, the levels of oxidative stress markers such as hydrogen peroxide (H O ) and superoxide (O ) increased dramatically after infection. The electrolyte leakage percentage (EL%), was found to be much greater in susceptible genotypes than resistant genotypes. The resistant gene SA14, which was found in genotypes Nebraska and Calypso at 800 bp, had an adequate level of resistance to bean rust with high grain yield potential. After infection, the transcriptions levels of and ) were higher in the resistant genotypes than susceptible genotypes. In conclusion, the resistant genotypes successfully displayed desirable agronomic traits and promising expectations in breeding programs for improving management strategies of common bean rust disease. The resistance was mediated by antioxidant enzymes, phenolic compounds, and defense gene expressions, as well as the resistant gene SA14.