Single-cell transcriptomics reveals specific RNA editing signatures in the human brain

• Published in: RNA (Cambridge) Vol. 23; no. 6; pp. 860 - 865
• Main Authors: Picardi, Ernesto, Horner, David S., Pesole, Graziano
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.06.2017
• Subjects: Astrocytes - metabolism; Brain - metabolism; Cluster Analysis; Databases, Nucleic Acid; Deamination; Editing; Gene Expression Profiling; Gene Expression Regulation; Humans; Neurons - metabolism; RNA Editing; Single-Cell Analysis - methods; Transcriptome; RNA editing; single cell; RNA-seq; transcriptome

While RNA editing by A-to-I deamination is a requisite for neuronal function in humans, it is under-investigated in single cells. Here we fill this gap by analyzing RNA editing profiles of single cells from the brain cortex of living human subjects. We show that RNA editing levels per cell are bimod...