Expression of clpX, an ATPase Subunit of the Clp Protease, Is Heat and Cold Shock Inducible in Lactococcus lactis

• Published in: Journal of dairy science Vol. 84; no. 8; pp. 1783 - 1785
• Main Authors: Skinner, M.M., Trempy, J.E.
• Format: Journal Article
• Language: English
• Published: Savoy, IL Elsevier Inc 01.08.2001; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: Adenosine Triphosphatases; Amino Acid Sequence; ATPase; ATPases Associated with Diverse Cellular Activities; Bacterial Proteins - genetics; Base Sequence; Biological and medical sciences; ClpX; cold shock; Cold Temperature; Cryopreservation; Endopeptidase Clp; Escherichia coli Proteins; Food industries; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial - physiology; Genes, Bacterial - genetics; heat shock; Heat-Shock Proteins - genetics; Hot Temperature; Lactococcus lactis; Lactococcus lactis - genetics; Milk and cheese industries. Ice creams; Molecular Chaperones; Molecular Sequence Data; protease; Serine Endopeptidases; protease; ClpX; Lactococcus lactis; ORF; ATPase; cold shock; heat shock; Lactic acid bacteria; Enzyme; Applied microbiology; Adenosinetriphosphatase; Experimental study; Gene expression; Subunit; Streptococcaceae; Peptidases; Gene; Thermal shock; Dairy industry; Bacteria; Hydrolases; Micrococcales; Molecular cloning
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(01)74615-2

In this study, the clpX gene and surrounding sequences were cloned and sequenced from Lactococcus lactis. The putative clpX gene encodes a 411 amino acid polypeptide with a predicted molecular weight of 45.8 kDa. Analysis of the relative levels of clpX transcript revealed that in addition to a role in proteolysis of heat damaged proteins, ClpX may also be involved in cryo-protection.