Within-Host Competition between Two Entomopathogenic Fungi and a Granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)

• Published in: Insects (Basel, Switzerland) Vol. 9; no. 2; p. 64
• Main Authors: Pauli, Giuliano, Moura Mascarin, Gabriel, Eilenberg, Jørgen, Delalibera Júnior, Italo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.06.2018; MDPI
• Subjects: Beauveria bassiana; beta-baculovirus; Bioassays; Borers; Cadavers; Competition; Crambidae; Diatraea saccharalis; Entomopathogenic fungi; Fungi; Infections; Inoculation; Inoculum; Insecticides; Insects; Laboratories; Larvae; Lepidoptera; Metarhizium anisopliae; mixed infection; Pathogens; Reproduction; Sporulation; Sugarcane; sugarcane borer; Virulence; Viruses; Brazil; United States > US; Beauveria bassiana; sugarcane borer; mixed infection; virulence; Metarhizium anisopliae; beta-baculovirus
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects9020064

We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer (Fabricius) (Lepidoptera: Crambidae). In our model system, we highlight the antagonistic effects of the co-inoculation of and granulovirus (DisaGV) on virulence, compared to their single counterparts. By contrast, combinations of and , or and DisaGV, have resulted in additive effects against the insect. Intriguingly, most cadavers that were derived from dual or triple infections, produced signs/symptoms of only one species after the death of the infected host. In the combination of fungi and DisaGV, there was a trend where a higher proportion of viral infection bearing conspicuous symptoms occurred, except when the larvae were inoculated with and DisaGV at the two highest inoculum rates. Co-infections with and did not affect pathogen reproduction, since the sporulation from co-inoculated larvae did not differ from their single counterparts.