Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells

• Published in: Biochemical and biophysical research communications Vol. 372; no. 1; pp. 230 - 235
• Main Authors: Rich, Jason T., Rosová, Ivana, Nolta, Jan A., Myckatyn, Terence M., Sandell, Linda J., McAlinden, Audrey
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.07.2008
• Subjects: Adipogenesis - genetics; Adipose Tissue - cytology; Adipose Tissue - drug effects; Adult; Alkaline Phosphatase - genetics; CCAAT-Enhancer-Binding Protein-alpha - genetics; Chondrocytes - metabolism; Chondrogenesis; Chondrogenesis - genetics; Collagen Type II - genetics; Collagen Type X - genetics; Core Binding Factor Alpha 1 Subunit - genetics; DNA-Binding Proteins - genetics; Female; Gene Expression Profiling; Hedgehog Proteins - genetics; High Mobility Group Proteins - genetics; Human adipose-derived stromal cells (HADSC); Humans; In vitro differentiation; Male; Mesenchymal stem cells; Mesenchymal stromal cell (MSC); Nuclear Proteins - genetics; Osteogenesis - genetics; Osterix; PPAR gamma - genetics; RNA, Messenger - analysis; RNA, Messenger - metabolism; Runx2; SOX9 Transcription Factor; SOXD Transcription Factors; Sp7 Transcription Factor; Stromal Cells - cytology; Stromal Cells - drug effects; TGF-β3; Transcription factors; Transcription Factors - genetics; Transforming Growth Factor beta3 - pharmacology; Up-Regulation; Human adipose-derived stromal cells (HADSC); Transcription factors; Mesenchymal stromal cell (MSC); In vitro differentiation; Osterix; TGF-β3; Chondrogenesis; Mesenchymal stem cells; Runx2
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.05.022

The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.