Utilization of a novel autologous killed tri-vaccine (serogroups B [Typhimurium], C [Mbandaka] and E [Orion]) for Salmonella control in commercial poultry breeders

• Published in: Avian pathology Vol. 39; no. 1; pp. 31 - 39
• Main Authors: Pavic, Anthony, Groves, Peter J, Cox, Julian M
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01.02.2010; Taylor & Francis Ltd
• Subjects: Animal Husbandry; animal pathogenic bacteria; Animals; Antibodies, Bacterial; Australia; autologous vaccines; broiler breeders; Chickens; disease control; disease incidence; disease reservoirs; egg yolk; Egg Yolk - immunology; enzyme-linked immunosorbent assay; Female; hens; immune response; Immunity; Immunization; Immunization Schedule; Immunoassay; inactivated vaccines; Male; maternal immunity; microbial colonization; Poultry; poultry diseases; Poultry Diseases - immunology; Poultry Diseases - microbiology; Poultry Diseases - prevention & control; Salmonella; Salmonella enterica subsp. enterica serovar Agona; Salmonella enterica subsp. enterica serovar Infantis; Salmonella enterica subsp. enterica serovar Mbandaka; Salmonella enterica subsp. enterica serovar Orion; Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella enterica subsp. enterica serovar Zanzibar; Salmonella Infections, Animal - immunology; Salmonella Infections, Animal - microbiology; Salmonella Infections, Animal - prevention & control; Salmonella typhimurium; Salmonella typhimurium - immunology; Salmonella Vaccines - immunology; serotypes; temporal variation; trivalent vaccines; vaccination; Vaccines; Vaccines, Inactivated - immunology; Australia
• ISSN: 0307-9457; 1465-3338
• DOI: 10.1080/03079450903454277

An autologous killed trivalent vaccine (3x10⁸ colony-forming units [CFU]), based on three Salmonella serovars (Typhimurium - serogroup B, Mbandaka - serogroup C, and Orion - serogroup E) prevalent in the flocks of Australian poultry companies, was developed using Salenvac techniques. At 20 weeks, hens vaccinated at 12 and 17 weeks as well as non-vaccinated hens were challenged (250 µl of 10⁷ CFU) with autologous and heterologous serovars belonging to serogroup B (Typhimurium and Agona), serogroup C (Mbandaka and Infantis) and serogroup E (Orion and Zanzibar). Overall, vaccination resulted in a significant difference in carriage of Salmonella between non-vaccinated and vaccinated commercial Cobb hens (P <0.05) for serogroups B and C. However, due to low colonization rates in the non-vaccinated birds, no significant difference (P>0.05) could be determined for serogroup E. All vaccinated flocks produced a significant antibody response (P<0.001) to the S. Typhimurium vaccine strain, measured using a S. Typhimurium enzyme-linked immunosorbent assay (Guildhay), which peaked at 20 weeks of age, with 39% of the hens positive. Maternal antibodies were detected in 16% of the yolks from eggs produced by these flocks. There was a significant difference after challenge with Salmonella (P <0.05) among 1-day-old chicks from vaccinated versus non-vaccinated parents, when challenged using 10⁴ CFU but not when challenged with 10⁸ CFU. The success of this trial resulted in the incorporation of this vaccine into a Salmonella control system in commercial broiler breeder production.