Development and Characterization of 20 Genomic SSR Markers for Ornamental Cultivars of Weigela

(Caprifoliaceae) is a genus of ornamental plants popular for its phenotypic variation and hardiness, that includes species hybridized to produce the commercially available cultivars. Despite its popularity, limited genetic resources exist for the genus. Twenty genomic simple sequence repeat (gSSR) m...

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Published inPlants (Basel) Vol. 11; no. 11; p. 1444
Main Authors Hamm, Trinity P, Boggess, Sarah L, Kandel, Jinita Sthapit, Staton, Margaret E, Huff, Matthew L, Hadziabdic, Denita, Shoemaker, DeWayne, Adamczyk, Jr, John J, Nowicki, Marcin, Trigiano, Robert N
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 29.05.2022
MDPI
Subjects
Alleles
Caprifoliaceae
Coverage
Cultivars
Flowers & plants
Gene mapping
Gene sequencing
Genetic diversity
Genetic resources
Genetic structure
Genomes
Genomics
Germplasm
gSSR
Hybridization
Marker-assisted selection
Markers
microsatellites
Nucleotide sequence
Ornamental plants
Phenotypic variations
Phylogenetics
Plant breeding
Population genetics
Ribosomal DNA
Weigela
Whole genome sequencing
United States > US
Europe
microsatellites
genetic diversity
Caprifoliaceae
gSSR
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Summary:(Caprifoliaceae) is a genus of ornamental plants popular for its phenotypic variation and hardiness, that includes species hybridized to produce the commercially available cultivars. Despite its popularity, limited genetic resources exist for the genus. Twenty genomic simple sequence repeat (gSSR) markers distributed across the genome were developed using low coverage whole-genome sequencing data of Spilled Wine . A cross-amplification evaluation with these 20 gSSR markers on a collection of 18 cultivars revealed a total of 111 unique alleles, including 36 private alleles. A diagrammatic key was constructed to identify cultivars using only six of the gSSR markers, demonstrating the newly developed gSSR markers are immediately useful for cultivar identification. Future uses could include breeding with marker-assisted selection, determining the history of hybridization of the current cultivated lines, aiding in the construction of genetic maps, and assessing the patterns of population genetic structure of spp.
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These authors contributed equally to this work.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11111444