Sequence of the fusion protein gene of human parainfluenza type 2 virus and its 3′ intergenic region: Lack of small hydrophobic (SH) gene

• Published in: Virology (New York, N.Y.) Vol. 178; no. 1; pp. 289 - 292
• Main Authors: Kawano, Mitsuo, Bando, Hisanori, Ohgimoto, Shinji, Kondo, Kunio, Tsurudome, Masato, Nishio, Machiko, Ito, Yasuhiko
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1990; Elsevier
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; DNA, Viral - analysis; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genetics; Genomic Library; Humans; Introns; Microbiology; Molecular Sequence Data; mumps virus; Newcastle disease virus; Parainfluenza Virus 2, Human - genetics; Respirovirus - genetics; Retroviridae Proteins, Oncogenic - genetics; Sequence Homology, Nucleic Acid; Solubility; Viral Fusion Proteins - genetics; Virology; Virus; Proteins; Membrane fusion; Gene; Nucleotide sequence; Paramyxovirus 2; Comparative study; Paramyxovirus; Aminoacid sequence; Paramyxoviridae
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(90)90406-H

cDNA clones representing the fusion (F) gene of human parainfluenza virus type 2 (PIV-2) were isolated from cDNA libraries constructed from virus-specific mRNA and genomic RNA, and the complete nucleotide sequence of the F gene was determined. The F gene is 1854 nucleotides long and encodes one long open reading frame of 551 amino acids. The cleavage site for activation of the precursor F o protein is ThrArgGlnLysArg. The F gene of PIV-2 is most closely related to those of simian virus 5 (SV5) and mumps virus (MuV). Interestingly, although the HN glycoprotein of PIV-2 shows no relatedness to the HA glycoprotein of measles virus (MV), a distinct homology is found in the F proteins of PIV-2 and MV. As concerns F proteins, paramyxoviruses can be divided intt two subgroups; that is, PIV-2, SV5, and MuV belong to one group, and HPIV-1, SV, and PIV-3 belong to the other group. Newcastle disease virus (NDV) and MV are intermediate. Coding regions for small hydrophobic (SH) proteins have been found between the HN and F genes of SV5 and MuV, which are the viruses most closely related to PIV-2. However, such a gene could not be detected in two different strains of PIV-2.