Preparation of Biofilm Electrode with Xanthomonas sp . and Carbon Nanotubes and the Application to Rapid Biochemical Oxygen Demand Analysis in High-Salt Condition

• Published in: Water environment research Vol. 80; no. 8; pp. 699 - 702
• Main Authors: Chen, Jing, Yu, Zhiguo, Sun, Jinfeng, Jia, Jianbo, Li, Genxi
• Format: Journal Article
• Language: English
• Published: Alexandria, VA Water Environment Federation 01.08.2008; Blackwell Publishing Ltd
• Subjects: Applied sciences; Bacteria; Biochemical oxygen demand; Biofilms; Biological oxygen demand analysis; Biosensing techniques; carbon nanotube; Cells, Immobilized; Chemical plants; Demand analysis; Electrodes; Exact sciences and technology; Factories; General purification processes; high‐salt wastewater; immobilized microorganism; Membrane filters; Methods; Microelectrodes; Nanotubes, Carbon; Organic chemistry; Oxygen; Oxygen - analysis; Oxygen - metabolism; Pharmaceutical preparations; Pollution; Polyvinyl alcohol; Response time; Sensors; Standard deviation; Waste Disposal, Fluid; Wastewater; Wastewaters; Water - analysis; Water treatment and pollution; Xanthomonas; Xanthomonas - physiology; Electrodes; Drug; Response time; Preparation; Biofilm; Organic compounds; Waste water
• ISSN: 1061-4303; 1554-7531
• DOI: 10.2175/106143008X276732

A Xanthomonas sp. was isolated from the sludge on the drain outlet of a pharmaceutical factory. Then, the bacterium and carbon nanotubes (CNTs) were co-attached to an oxygen electrode for rapid analysis of biochemical oxygen demand (BOD). The response current was linear with BOD values in the range 10 to 300 mg/L for standard BOD solution with a response time of 35 seconds (R = 0.9994) and 20 to 580 mg/L for pharmaceutical waste water with a response time < 200 seconds (R = 0.9985), which means that this modified electrode might be used for online BOD analysis of pharmaceutical wastewater. Further studies revealed that the modified electrode can be used for BOD measurement in a high-salt condition. Also, the bacterium/CNTs biofilm can maintain its activity and good performance, even after being sealed and stored at 4°C for 50 days.