Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imaging

Ex vivo generated dendritic cells are currently used to induce therapeutic immunity in solid tumors. Effective immune response requires dendritic cells to home and remain in lymphoid organs to allow for adequate interaction with T lymphocytes. The aim of the current study was to detect and track Fer...

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Published inMagnetic resonance in medicine Vol. 64; no. 5; pp. 1510 - 1519
Main Authors Briley-Saebo, Karen C., Leboeuf, Marylene, Dickson, Stephen, Mani, Venkatesh, Fayad, Zahi A., Karolina Palucka, A., Banchereau, Jacques, Merad, Miriam
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.11.2010
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Summary:Ex vivo generated dendritic cells are currently used to induce therapeutic immunity in solid tumors. Effective immune response requires dendritic cells to home and remain in lymphoid organs to allow for adequate interaction with T lymphocytes. The aim of the current study was to detect and track Feridex labeled human dendritic cells in murine models using magnetic resonance imaging. Human dendritic cells were incubated with Feridex and the effect of labeling on dendritic cells immune function was evaluated. Ex vivo dendritic cell phantoms were used to estimate sensitivity of the magnetic resonance methods and in vivo homing was evaluated after intravenous or subcutaneous injection. R2*‐maps of liver, spleen, and draining lymph nodes were obtained and inductively coupled plasma mass spectrometry or relaxometry methods were used to quantify the Feridex tissue concentrations. Correlations between in vivo R2* values and iron content were then determined. Feridex labeling did not affect dendritic cell maturation or function. Phantom results indicated that it was possible to detect 125 dendritic cells within a given slice. Strong correlation between in vivo R2* values and iron deposition was observed. Importantly, Feridex‐labeled dendritic cells were detected in the spleen for up to 2 weeks postintravenous injection. This study suggests that magnetic resonance imaging may be used to longitudinally track Feridex‐labeled human dendritic cells for up to 2 weeks after injection. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Bibliography:NIH - No. RO1-CA078846; No. AR054083-01
ark:/67375/WNG-RH5KJT8N-B
Unknown funding agency - No. U19-A1057234
istex:437D4BDC256D1973C0E3E7BDB36F2C548DC2D66B
Baylor Health Care foundation
ArticleID:MRM22519
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0740-3194
1522-2594
1522-2594
DOI:10.1002/mrm.22519