Aptamer-Based Affinity Labeling of Proteins

A most able label: Labeled aptamers (see picture) can be cross‐linked to their target structures in a light‐dependent and highly specific manner as a result of a new strategy termed aptamer‐based affinity labeling (ABAL) of proteins. The aptamer–protein complexes can be enriched in vitro, from a cel...

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Published inAngewandte Chemie International Edition Vol. 51; no. 36; pp. 9176 - 9180
Main Authors Vinkenborg, Jan L., Mayer, Günter, Famulok, Michael
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 03.09.2012
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
Affinity
Affinity Labels - chemistry
affinity-based proteomic profiling (ABPP)
aptamers
Aptamers, Nucleotide - chemistry
Biological
Cell Line, Tumor
cell-SELEX
Cellular
Crosslinking
Humans
Labels
Marking
photo-affinity labeling
Pictures
Proteins
Proteins - chemistry
Proteins - metabolism
proteomics
Proto-Oncogene Proteins c-met - chemistry
Proto-Oncogene Proteins c-met - metabolism
SELEX Aptamer Technique
Strategy
Ultraviolet Rays
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Summary:A most able label: Labeled aptamers (see picture) can be cross‐linked to their target structures in a light‐dependent and highly specific manner as a result of a new strategy termed aptamer‐based affinity labeling (ABAL) of proteins. The aptamer–protein complexes can be enriched in vitro, from a cellular lysate and from the surface of living cells, opening new ways to study aptamer interactions in biological contexts.
Bibliography:This work was supported by the European Science Foundation ESF (Synapta), the Alexander von Humboldt Stiftung (J.L.V.), and the Deutsche Forschungsgemeinschaft (SFB 645). We thank A. Schmitz for discussions, V. Fieberg for technical support and K. Schopen for her assistance in characterizing the D17.4 aptamers.
istex:9F7BE5635ED8536BA37D180C4F5A3AB1CD0FEDA0
ark:/67375/WNG-PR1B6HZ9-R
ESF
Deutsche Forschungsgemeinschaft - No. SFB 645
European Science Foundation
ArticleID:ANIE201204174
Alexander von Humboldt Stiftung
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201204174