Oxyphytosterols are present in plasma of healthy human subjects

• Published in: British journal of nutrition Vol. 91; no. 1; pp. 101 - 106
• Main Authors: Grandgirard, André, Martine, Lucy, Demaison, Luc, Cordelet, Catherine, Joffre, Corinne, Berdeaux, Olivier, Semon, Etienne
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.01.2004; Cambridge University Press (CUP)
• Subjects: Adult; Cholesterol; Female; Food and Nutrition; Gas Chromatography-Mass Spectrometry - methods; Human plasma; Human subjects; Humans; Life Sciences; Male; Mass spectrometry; Middle Aged; Oxidation-Reduction; Oxyphytosterols; Phytosterols; Phytosterols - blood; Plasma; Scientific imaging; Sitosterols - blood; Sterols; France; United States > US; Human plasma; Mass spectrometry; Oxyphytosterols; Phytosterols
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1079/BJN20031025

The oxidised derivatives of phytosterols (oxyphytosterols) were identified in plasma samples from thirteen healthy human volunteers, using MS. All the samples contained noticeable quantities of (24R)-5β,6β-epoxy-24-ethylcholestan-3β-ol (β-epoxysitostanol) and (24R)-ethylcholestan-3β,5α,6β-triol (sitostanetriol) and also trace levels of (24R)-5α,6α-epoxy-24-ethylcholestan-3β-ol (α-epoxysitostanol), (24R)-methylcholestan-3β,5α,6β-triol (campestanetriol) and (24R)-ethylch olest-5-en-3β-ol-7-one(7-ketositosterol). The amounts of these oxyphytosterols in plasma varied from 4·8 to 57·2 ng/ml. There are two possibilities concerning the origin of these compounds. First, they could come from the small amounts of oxyphytosterols in food. Second, they could originate from the in vivo oxidation of phytosterols in plasma. Very few data actually exist concerning these compounds. Their identification in human samples suggests that further research is necessary in this field.