Veillonella montpellierensis sp. nov., a novel, anaerobic, Gram-negative coccus isolated from human clinical samples

• Published in: International journal of systematic and evolutionary microbiology Vol. 54; no. 4; pp. 1311 - 1316
• Main Authors: Jumas-Bilak, Estelle, Carlier, Jean-Philippe, Jean-Pierre, Helene, Teyssier, Corinne, Gay, Bernard, Campos, Josiane, Marchandin, Helene
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.2004; Society for General Microbiology; Microbiology Society
• Subjects: Adult; Amniotic Fluid - microbiology; Anaerobiosis; Bacterial Proteins - genetics; Bacteriology; Biological and medical sciences; DNA, Bacterial - chemistry; DNA, Ribosomal - chemistry; Fatty Acids - analysis; Female; Fundamental and applied biological sciences. Psychology; Gastric Juice - microbiology; Genes, rRNA; Gentian Violet; Gram-Negative Bacterial Infections - microbiology; Humans; Infant, Newborn; Life Sciences; Microbiology; Microbiology and Parasitology; Microscopy, Electron; Miscellaneous; Molecular Chaperones - genetics; Molecular Sequence Data; Phenazines; Phylogeny; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology; Systematics; Veillonella - classification; Veillonella - cytology; Veillonella - isolation & purification; Veillonella - physiology; Veillonella montpellierensis; Human; Veillonellaceae; Veillonella; Taxonomy; Bacteria; New species; Gram negative bacteria
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.02952-0

1 Laboratoire de Bactériologie–Virologie, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, 34060 Montpellier Cedex 5, France 2 Centre National de Référence des Bactéries Anaérobies et du Botulisme, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France 3 Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France Correspondence Hélène Marchandin h-marchandin{at}chu-montpellier.fr Three strains of a hitherto unknown, Gram-negative, anaerobic coccus were isolated from human samples. At the phenotypic level, the isolates displayed all the characteristics of bacteria belonging to the genus Veillonella . Sequence analysis revealed that the three strains shared >99·5 % similarity in 16S rDNA sequence and >98·4 % similarity in dnaK sequence. The three unknown strains formed a separate subclade that was clearly remote from Veillonella species of human and animal origin. Based on these results, the three strains were considered to represent a novel species within the genus Veillonella , for which the name Veillonella montpellierensis is proposed. The type strain of the species is ADV 281.99 T (=CIP 107992 T =CCUG 48299 T ). Abbreviations: EM, electron microscopy; ML, maximum-likelihood; NJ, neighbour-joining The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of Veillonella montpellierensis ADV 281.99 T (=CIP 107992 T =CCUG 48299 T ) is AF473836 .