Maternal protein restriction during gestation impairs female offspring pancreas development in the rat

• Published in: Nutrition research (New York, N.Y.) Vol. 36; no. 8; pp. 855 - 862
• Main Authors: Calzada, Lizbeth, Morales, Angélica, Sosa-Larios, Tonantzin C, Reyes-Castro, Luis A, Rodríguez-González, Guadalupe L, Rodríguez-Mata, Verónica, Zambrano, Elena, Morimoto, Sumiko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2016
• Subjects: Animal Nutritional Physiological Phenomena; Animals; Developmental programming; Diet, Protein-Restricted - adverse effects; Female; Gastroenterology and Hepatology; Glucagon-Secreting Cells - cytology; Insulin-Secreting Cells - cytology; Insulin-Secreting Cells - physiology; Islets of Langerhans - cytology; Islets of Langerhans - growth & development; Lactation; Langerhans islets; Maternal nutrition; Maternal Nutritional Physiological Phenomena; Pancreas - growth & development; Pregnancy; Prenatal Exposure Delayed Effects; Protein malnutrition; Rat; Rats; Rats, Wistar; Sexual Maturation; Weaning; PBS; Langerhans islets; C; rat; LP; DAB; PND; low protein; control; protein malnutrition; NOM; BSA; analysis of variance; 3,3′- diaminobenzidine tetrahydrochloride; ANOVA; phosphate buffered saline; postnatal day; SEM; standard error of the mean; maternal nutrition; official Mexican guideline; bovine serum albumin; developmental programming; Rat; Developmental programming; Protein malnutrition; Maternal nutrition
• ISSN: 0271-5317; 1879-0739
• DOI: 10.1016/j.nutres.2016.03.007

Abstract A maternal low-protein (LP) diet programs fetal pancreatic islet beta cell development and function and predisposes offspring to metabolic dysfunction later in life. We hypothesized that maternal protein restriction during pregnancy differentially alters beta and alpha cell populations in offspring by modifying islet ontogeny and function throughout life. We aimed to investigate the effect of an LP maternal diet on pancreatic islet morphology and cellular composition in female offspring on postnatal day (PND) 7, 14, 21, 36 and 110. Mothers were divided into two groups: during pregnancy, the control group (C) was fed a diet containing 20% casein, and the LP group was fed an isocaloric diet with 10% casein. Offspring pancreases were obtained at each PND and then processed. Beta and alpha cells were detected by immunohistochemistry, and cellular area and islet size were quantified. Islet cytoarchitecture and total area were similar in C and LP offspring at all ages studied. At the early ages (PND 7–21), the proportion of beta cells was lower in LP than C offspring. The proportion of alpha cells was lower in LP than C offspring on PND 14 and higher on PND 21. The beta/alpha cell ratio was lower in LP compared to C offspring on PND 7 and 21 and higher on PND 36 (being similar on PNDs 14 and 110). We concluded that maternal protein restriction during pregnancy modifies offspring islet cell ontogeny by altering the proportions of islet sizes and by reducing the number of beta cells postnatally, which may impact pancreatic function in adult life.