Solid-state NMR spectroscopy of a membrane protein in biphenyl phospholipid bicelles with the bilayer normal parallel to the magnetic field

• Published in: Journal of magnetic resonance (1997) Vol. 193; no. 1; pp. 133 - 138
• Main Authors: Park, Sang Ho, Loudet, Cécile, Marassi, Francesca M., Dufourc, Erick J., Opella, Stanley J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2008
• Subjects: Aligned sample; Bacteriophage Pf1 - chemistry; Biphenyl Compounds - chemistry; Lipid Bilayers - chemistry; Membrane protein; Nitrogen Isotopes; Nuclear Magnetic Resonance, Biomolecular - methods; Phospholipids - chemistry; Solid-state NMR; TBBPC bicelles; Solid-state NMR; Membrane protein; TBBPC bicelles; Aligned sample
• ISSN: 1090-7807; 1096-0856
• DOI: 10.1016/j.jmr.2008.04.033

Bicelles composed of the long-chain biphenyl phospholipid TBBPC (1-tetradecanoyl-2-(4-(4-biphenyl)butanoyl)-sn-glycero-3-PC) and the short-chain phospholipid DHPC align with their bilayer normals parallel to the direction of the magnetic field. In contrast, in typical bicelles the long-chain phospholipid is DMPC or DPPC, and the bilayers align with their normals perpendicular to the field. Samples of the membrane-bound form of the major coat protein of Pf1 bacteriophage in TBBPC bicelles are stable for several months, align magnetically over a wide range of temperatures, and yield well-resolved solid-state NMR spectra similar to those obtained from samples aligned mechanically on glass plates or in DMPC bicelle samples “flipped” with lanthanide ions so that their bilayer normals are parallel to the field. The order parameter of the TBBPC bicelle sample decreases from approximately 0.9 to 0.8 upon increasing the temperature from 20 °C to 60 °C. Since the frequency spans of the chemical shift and dipolar coupling interactions are twice as large as those obtained from proteins in DMPC bicelles without lanthanide ions, TBBPC bicelles provide an opportunity for structural studies with higher spectral resolution of the metal-binding membrane proteins without the risk of chemical or spectroscopic interference from the added lanthanide ions. In addition, the large temperature range of these samples is advantageous for the studies of membrane proteins that are unstable at elevated temperatures and for experiments requiring measurements as a function of temperature.