Groups of p53 target genes involved in specific p53 downstream effects cluster into different classes of DNA binding sites

• Published in: Oncogene Vol. 21; no. 51; pp. 7901 - 7911
• Main Authors: HUA QIAN, TING WANG, NAUMOVSKI, Louie, LOPEZ, Charles D, BRACHMANN, Rainer K
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 07.11.2002; Nature Publishing Group
• Subjects: Apoptosis; Apoptosis - genetics; Binding Sites; Binding sites (Biochemistry); Biological and medical sciences; Cell cycle; Cell Cycle - genetics; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Consensus Sequence; Deoxyribonucleic acid; DNA; DNA - metabolism; DNA Damage; DNA repair; DNA Repair - genetics; DNA-ligand interactions; Fundamental and applied biological sciences. Psychology; Fungal Proteins - biosynthesis; Fungal Proteins - genetics; Gene Expression Profiling; Gene regulation; Genes, p53; Genes, Reporter; Growth factors; Hematology; Humans; Luciferases - biosynthesis; Luciferases - genetics; MDM2 protein; Medicine; Molecular and cellular biology; Oncology; p53 Protein; Physiological aspects; Plasmids - genetics; Proteins; Recombinant Fusion Proteins - biosynthesis; Saccharomyces cerevisiae - metabolism; Transcription; Transfection; Tumor suppressor genes; Tumor Suppressor Protein p53 - physiology; Tumors; United States; Oregon; United States > US; California; Yeast; Genomics; Binding site; Malignant tumor; Gene expression; DNA binding site; DNA repair; p53; Vertebrata; Regulation(control); TP53 Gene; Cell line; Mammalia; DNA; Cell cycle; Lesion; Onc gene; Apoptosis
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1205974

The tumor suppressor protein p53, once activated, can cause either cell cycle arrest or apoptosis through transactivation of target genes with p53 DNA binding sites (DBS). To investigate the role of p53 DBS in the regulation of this profound, yet poorly understood decision of life versus death, we systematically studied all known and potential p53 DBS. We analysed the DBS separated from surrounding promoter regions in yeast and mammalian assays with and without DNA damage. p53 efficiently utilized the DBS of MDM2 and of genes connected to cell cycle arrest, DNA repair and the death receptor pathway of apoptosis. However, p53 was unable to utilize two-thirds of the isolated DBS, a subset that included almost all DBS of apoptosis-related genes. Neither ASPP2, a p53-interacting protein reported to specifically stimulate p53 transcriptional activity on apoptosis-related promoters, nor DNA damage resulted in p53 utilization of isolated DBS of apoptosis-related genes. Thus, a major regulation of p53 activity occurs at the level of p53 DBS themselves by posing additional requirements for the successful utilization of apoptosis-related DBS.