Parabacteroides distasonis Properties Linked to the Selection of New Biotherapeutics

• Published in: Nutrients Vol. 14; no. 19; p. 4176
• Main Authors: Chamarande, Jordan, Cunat, Lisiane, Pavlov, Nadine, Alauzet, Corentine, Cailliez-Grimal, Catherine
• Format: Journal Article Publication
• Language: English
• Published: Basel MDPI AG 01.10.2022; MDPI
• Subjects: Anti-Inflammatory Agents; Bacteria; Bacteroidetes; Caco-2 Cells; Cytokines; dysbiosis; gut microbiota; Health promotion; Humans; Immune system; Immunomodulation; Inflammatory bowel diseases; Intestinal microflora; Laboratories; Leukocytes (mononuclear); Life Sciences; Lipopolysaccharides; Microbiomes; Microbiota; new biotherapeutic product (NBP); Parabacteroides distasonis; Peripheral blood mononuclear cells; Ulcerative colitis; France; Parabacteroides distasonis; immunomodulation; new biotherapeutic product (NBP); gut microbiota; dysbiosis
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14194176

Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis. In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs). Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host. In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)). This approach allowed for the identification of five strains that combined almost all the in vitro biotherapeutic properties tested. However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines. Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.