Identification of Key Genes Regulating Sorghum Mesocotyl Elongation through Transcriptome Analysis

• Published in: Genes Vol. 14; no. 6; p. 1215
• Main Authors: Ju, Lan, Lv, Na, Yin, Feng, Niu, Hao, Yan, Haisheng, Wang, Yubin, Fan, Fangfang, Lv, Xin, Chu, Jianqiang, Ping, Junai
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.06.2023; MDPI
• Subjects: Abscisic Acid; Agricultural production; Analysis; Cell cycle; Cell division; Cell walls; Cytokinins; Edible Grain - genetics; Elongation; Energy metabolism; Gene Expression Profiling; Genes; Genomes; Molecular modelling; Phytohormones; Plant Growth Regulators - genetics; Plant Growth Regulators - metabolism; Salicylic acid; Seedlings; Signal transduction; Software; Sorghum; Sorghum - metabolism; Transcription factors; Transcriptomes; Zeatin; United States > US; Japan; mesocotyl; sorghum; transcriptome analysis; key gene
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes14061215

Sorghum with longer mesocotyls is beneficialfor improving its deep tolerance, which is important for the seedling rates. Here, we perform transcriptome analysis between four different sorghum lines, with the aim of identifying the key genes regulating sorghum mesocotyl elongation. According to the mesocotyl length (ML) data, we constructed four comparison groups for the transcriptome analysis and detected 2705 common DEGs. GO and KEGG enrichment analysis showed that the most common category of DEGs were involved in cell wall, microtubule, cell cycle, phytohormone, and energy metabolism-related pathways. In the cell wall biological processes, the expression of , , , , and are increased in the sorghum lines with long ML. In the plant hormone signaling pathway, five auxin-responsive genes and eight cytokinin/zeatin/abscisic acid/salicylic acid-related genes showed a higher expression level in the long ML sorghum lines. In addition, five ERF genes showed a higher expression level in the sorghum lines with long ML, whereas two ERF genes showed a lower expression level in these lines. Furthermore, the expression levels of these genes were further analyzed using real-time PCR (RT-qPCR), which showed similar results. This work identified the candidate gene regulating ML, which may provide additional evidence to understand the regulatory molecular mechanisms of sorghum mesocotyl elongation.