Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 11; no. 3; pp. 177 - 183
• Main Authors: Doster, Alan R., University of Nebraska-Lincoln, Nebraska, USA, Subramaniam, Sakthivel, University of Nebraska-Lincoln, Nebraska, USA, Yhee, J.Y., Konkuk University, Seoul, Republic of Korea, Kwon, B.J., University of Nebraska-Lincoln, Nebraska, USA, Yu, C.H., Konkuk University, Seoul, Republic of Korea, Kwon, S.Y., Konkuk University, Seoul, Republic of Korea, Osorio, Fernando A., University of Nebraska-Lincoln, Nebraska, USA, Sur, J.H., Konkuk University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 01.09.2010; The Korean Society of Veterinary Science
• Subjects: Animals; Circoviridae Infections - immunology; Circoviridae Infections - pathology; Circoviridae Infections - veterinary; Circovirus - immunology; Gene Expression Regulation - immunology; immunohistochemistry; Immunohistochemistry - veterinary; Interleukin-10 - immunology; Interleukin-10 - metabolism; interlukin-10; LINFOCITOS; LYMPHOCYTE; LYMPHOCYTES; Lymphoid Tissue - immunology; Lymphoid Tissue - metabolism; Lymphoid Tissue - pathology; Original; porcine circovirus type 2; Porcine Postweaning Multisystemic Wasting Syndrome - immunology; Porcine Postweaning Multisystemic Wasting Syndrome - pathology; Republic of Korea; Swine; T-Lymphocytes - immunology; 수의학; T-cells; porcine circovirus type 2; B-cells; immunohistochemistry; interlukin-10
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2010.11.3.177

Distribution and characterization of interlukin-10 (IL-10)-secreting cells in lymphoid tissues of pigs naturally infected with porcine circovirus type 2 (PCV2) were evaluated in accordance with PCV2 antigen detection. After screening a total of 56 pigs showing the symptoms of postweaning multisystemic wasting syndrome (PMWS), 15 pigs were PCV2 positive and 5 pigs, which showed stronger positive signals over multiples tissues were further investigated. This study showed that in PCV2-infected lymphoid tissues, particularly mandibular lymph node, spleen and tonsil, IL-10 expression was mainly localized in T-cell rich areas but rarely in B cell rich areas. IL-10 was highly expressed in bystander cells but rarely in PCV2-infected cells. Elevated IL-10 expression was predominantly associated with T cells, but rarely with B cells or with macrophages. The results of this study provide evidence for the role of IL-10 in chronic PCV2 infection and its relation to PCV2 antigen in affected tissues. Constantly elevated levels of IL-10 lead to immunosuppression in persistent and chronic viral infections. The increased IL-10 expression observed in PCV2 infection in this study suggests that IL-10-mediated immunosuppression may play an important role in the pathogenesis and maintenance of naturally occurring PCV2 infection.