Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering

• Published in: International journal of nanomedicine Vol. 6; no. default; pp. 2133 - 2141
• Main Authors: Hajiali, Hadi, Shahgasempour, Shapour, Naimi-Jamal, M Reza, Peirovi, Habibullah
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Press 01.01.2011; Dove Medical Press
• Subjects: Biocompatible Materials - chemistry; biocompatible scaffold; Calorimetry, Differential Scanning; Cell Adhesion - drug effects; Cell Survival - drug effects; Cells, Cultured; Elastic Modulus; gelatin; Gelatin - chemistry; Gelatin - pharmacology; Human Umbilical Vein Endothelial Cells - cytology; Humans; nanofiber; Nanofibers - chemistry; Nanotechnology; Original Research; polyglycolic acid; Polyglycolic Acid - chemistry; Polyglycolic Acid - pharmacology; Spectroscopy, Fourier Transform Infrared; Tensile Strength; Tissue Engineering; Tissue Scaffolds - chemistry; Umbilical Arteries - cytology; vascular tissue engineering; vascular tissue engineering; biocompatible scaffold; polyglycolic acid; gelatin; nanofiber
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/ijn.s24312

In this study, gelatin was blended with polyglycolic acid (PGA) at different ratios (0, 10, 30, and 50 wt%) and electrospun. The morphology and structure of the scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The mechanical properties were also measured by the tensile test. Furthermore, for biocompatibility assessment, human umbilical vein endothelial cells and human umbilical artery smooth muscle cells were cultured on these scaffolds, and cell attachment and viability were evaluated. PGA with 10 wt% gelatin enhanced the endothelial cells whilst PGA with 30 wt% gelatin increased smooth muscle cell adhesion, penetration, and viability compared with the other scaffold blends. Additionally, with the increase in gelatin content, the mechanical properties of the scaffolds were improved due to interaction between PGA and gelatin, as revealed by Fourier transform infrared spectroscopy and differential scanning calorimetry. Incorporation of gelatin improves the biological and mechanical properties of PGA, making promising scaffolds for vascular tissue engineering.