An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length

• Published in: Methods and protocols Vol. 3; no. 2; p. 27
• Main Authors: Joglekar, Mugdha V., Satoor, Sarang N., Wong, Wilson K.M., Cheng, Feifei, Ma, Ronald C.W., Hardikar, Anandwardhan A.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.04.2020; MDPI
• Subjects: cells; DNA; DNA replication; Observations; Physiological aspects; Protocol; real-time PCR; relative telomere length; Telomeres; Germany; Telomeres; relative telomere length; cells; real-time PCR; DNA

Telomeres represent the nucleotide repeat sequences at the ends of chromosomes and are essential for chromosome stability. They can shorten at each round of DNA replication mainly because of incomplete DNA synthesis of the lagging strand. Reduced relative telomere length is associated with aging and...