Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill

• Published in: Yàowu shi͡p︡in fenxi Vol. 26; no. 2; pp. 678 - 687
• Main Authors: Wang, Hsueh-Ting, Yang, Li-Chan, Yu, Hui-Ching, Chen, Miaw-Ling, Wang, Huei-Ju, Lu, Ting-Jang
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier B.V 01.04.2018; Food and Drug Administration; Taiwan Food and Drug Administration
• Subjects: Acidic polysaccharide; Acids; Agaricus - chemistry; Agaricus - metabolism; Agaricus blazei; Agaricus blazei Murill; Animals; Antigens; Arabinose; Barium; Biological activity; Carbohydrates; Chromatography; D-Galactose; D-Galacturonic acid; Enzyme-linked immunosorbent assay; Enzymes; Fermentation; Fucose; Fucose - analysis; Fucose - metabolism; Galactan; Galactose; Glucose; Glucose - metabolism; Immune system; Immunoglobulins; Investigations; L-Rhamnose; Macrophages; Macrophages - drug effects; Macrophages - immunology; Mannose; Methylation; Mice; Molecular weight; Monoclonal antibodies; NF-kappa B - genetics; NF-kappa B - immunology; NF-κB protein; NMR; Nuclear magnetic resonance; Original; Pattern recognition; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - metabolism; Polysaccharides - pharmacology; Proteins; RAW 264.7 Cells; Residues; Rhamnose; Saccharides; Sugar; TLR4 protein; TNF-α stimulation; Toll-like receptors; Tumor necrosis factor; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Tumor necrosis factor-α; Xylose; Japan; Taiwan; Acidic polysaccharide; TNF-α stimulation; Agaricus blazei Murill; Galactan; Fucose
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2017.07.006

Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.5 × 105 Da. The major sugars were l-fucose, l-arabinose, d-galactose, d-xylose, and d-galacturonic acid in the molar ratio of 6.4, 15.5, 28.5, 14.7, and 25.0% with a small amount of d-glucose, d-mannose, l-rhamnose, and d-glucuronic acid. Results indicated that the bioactive polysaccharides consisted of a (1,4)-Galp and (1,4)-GalAp back bone; (1,2)-Xyl and (1,2)-Rha might also comprise backbone or constitute side chain; linkage (1,5)-Ara and terminal fucosyl residues were also involved in the polysaccharides. Regarding bioactivity, removal of the terminal l-fucosyl residues reduced the TNF-α cytokine stimulating activity of the polysaccharides in a RAW 264.7 macrophage cell-line test, whereas NF-κB and TLR4 affected the polysaccharide-induced TNF-α production. [Display omitted] •This study investigated the polysaccharide profile from submerged fermentation of Agaricus blazei Murill.•Fucose-containing acidic polysaccharides designated as F3_FA were characterized.•F3_FA polysaccharides are the major bioactive component to induce TNF-α secretion from RAW264.7 macrophages.•Terminal α-L-fucose residues play an important role of bioactivity of the polysaccharides.•F3_FA polysaccharides induced cell bioactivity affected by TLR4 receptor.