Isolation of a cDNA encoding a human rolipram-sensitive cyclic AMP phosphodiesterase (PDE IV D)

• Published in: Gene Vol. 138; no. 1; pp. 253 - 256
• Main Authors: Baecker, Preston A., Obernolte, Rena, Bach, Chinh, Yee, Calvin, Shelton, Earl R.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 28.01.1994; Amsterdam Elsevier; New York, NY
• Subjects: 3',5'-Cyclic-AMP Phosphodiesterases - biosynthesis; 3',5'-Cyclic-AMP Phosphodiesterases - genetics; 3',5'-Cyclic-AMP Phosphodiesterases - metabolism; 3',5'-cyclic-nucleotide phosphodiesterase; Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; cDNA; Cloning, Molecular; DNA, Complementary - isolation & purification; Escherichia coli; Female; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Library; genes; Genes. Genome; heart; Heart library; Humans; Isoenzymes - biosynthesis; Isoenzymes - genetics; Isoenzymes - metabolism; man; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Muscles - enzymology; Myocardium - enzymology; nucleotide sequence; Organ Specificity; Phosphodiesterase Inhibitors - pharmacology; Polymerase Chain Reaction; prediction; Pregnancy; Pyrrolidinones - pharmacology; rat homolog; Rats; RNA, Messenger - biosynthesis; RNA, Messenger - metabolism; Rolipram; sensitive; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; skeletal tissue; aa; skeletal tissue; A; IC 50; ORF; SSC; nt; cGMP; PDE IV D; cAMP; h or h; bp; p; kb; rat homolog; cDNA; r or r; Heart library; PDE IV; Human; 3',5'-Cyclic-nucleotide phosphodiesterase; Nucleotide sequence; Enzyme; Tissue specificity; Esterases; Phosphoric diester hydrolases; Gene expression; Striated muscle; Complementary DNA; Hydrolases; Aminoacid sequence; Structural analysis
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(94)90818-4

cDNAs encoding human family-IV phosphodiesterase, subtype D (hPDE IV D) were isolated from a human heart cDNA library. The overlapping cDNAs encode a polypeptide of 604 amino acids (aa) with a predicted 0 M r of 68 502, which is 91.4% identical to the rat homolog, rPDE IV D. hPDE IV D produced in Escherichia coli was inhibited by rolipram. Expression of the hPDE IV D mRNA is widespread in human tissues and most abundant in skeletal muscle.