A portable laser photostimulation and imaging microscope
We describe a compact microscope that uses a spatial light modulator (SLM) to control the excitation laser light. The flexibility of SLMs, which can mimic virtually any optical transfer function, enables the experimenter to create, in software, arbitrary spatio-temporal light patterns, including foc...
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Published in | Journal of neural engineering Vol. 7; no. 4; p. 045001 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
IOP Publishing
01.08.2010
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Subjects | |
Online Access | Get full text |
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Summary: | We describe a compact microscope that uses a spatial light modulator (SLM) to control the excitation laser light. The flexibility of SLMs, which can mimic virtually any optical transfer function, enables the experimenter to create, in software, arbitrary spatio-temporal light patterns, including focusing and beam scanning, simply by calculating the appropriate phase mask. Our prototype, a scan-less device with no moving parts, can be used for laser imaging or photostimulation, supplanting the need for an elaborate optical setup. As a proof of principle, we generate complex excitation patterns on fluorescent samples and also perform functional imaging of neuronal activity in living brain slices. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1741-2552 1741-2560 1741-2552 |
DOI: | 10.1088/1741-2560/7/4/045001 |