Secretory proteins of the lung in rodents: immunocytochemistry

• Published in: The journal of histochemistry and cytochemistry Vol. 33; no. 6; pp. 564 - 568
• Main Authors: Singh, G, Katyal, SL, Ward, JM, Gottron, SA, Wong-Chong, ML, Riley, EJ
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.06.1985; SAGE Publications; Histochemical Society
• Subjects: Air breathing; Animals; Apoproteins - analysis; Apoproteins - immunology; Biological and medical sciences; Collodion; Cricetinae; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Histocytochemistry; Immunoenzyme Techniques; Lung - analysis; Mesocricetus; Mice; Pulmonary Surfactants - analysis; Pulmonary Surfactants - immunology; Rabbits; Rats; Rats, Inbred F344; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics; Vertebrates: respiratory system; Rat; Immunocytochemistry; Secretion; Interspecific comparison; Lung; Rodentia; Respiratory system; Antigen; Proteins; Clear cell; Vertebrata; Mammalia; Mouse; Secretory protein; Comparative study; Hamster
• ISSN: 0022-1554; 1551-5044
• DOI: 10.1177/33.6.3889141

The reactivity of rabbit antisera to rat lung secretory proteins with other rodent species was evaluated by immunocytochemistry. Rabbit anti-rat surfactant apoprotein antiserum reacts with the cytoplasm of rat, mouse, and hamster type II pneumocytes and is specific for these cells. Rabbit antiserum to rat Clara cell secretory proteins stains rat, mouse, and hamster Clara cells. Rabbit antisera specific to the two antigenic types of rat Clara cell antigens were also both reactive with rat, mouse, and hamster Clara cells. An antiserum to the non-serum proteins of hamster lung lavage was also prepared and shown to be specifically reactive with hamster Clara cells. The availability of specific reagents for secretory proteins of rodent lungs is expected to facilitate studies of the respective cell types in various pathologic states.