Tocotrienols from palm oil as potent inhibitors of lipid peroxidation and protein oxidation in rat brain mitochondria

• Published in: Neuroscience letters Vol. 195; no. 3; pp. 179 - 182
• Main Authors: Kamat, J.P., Devasagayam, T.P.A.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 11.08.1995; Elsevier
• Subjects: Animals; Antioxidants - pharmacology; Biochemistry and metabolism; Biological and medical sciences; Brain - metabolism; Brain - ultrastructure; Brain mitochondria; Central nervous system; Dietary Fats, Unsaturated - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Lipid peroxidation; Lipid Peroxidation - drug effects; Mitochondria - metabolism; Natural antioxidant; Oxidation-Reduction - drug effects; Palm Oil; Plant Oils - pharmacology; Protein carbonyls; Proteins - metabolism; Rats; Rats, Wistar; Tocotrienols; Vertebrates: nervous system and sense organs; Vitamin E - analogs & derivatives; Vitamin E - pharmacology; Lipid peroxidation; Brain mitochondria; Palm oil; Natural antioxidant; Tocotrienols; Protein carbonyls; Vertebrata; Mitochondria; Mammalia; Rat; Rodentia; Central nervous system; Lipids; α-Tocopherol; Antioxidant; Brain (vertebrata); Peroxidation
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/0304-3940(95)11812-B

The tocotrienol-rich-fraction (TRF) from palm oil, being tried as a more economical and efficient substitute for α-tocopherol, significantly inhibited oxidative damage in vitro to both lipids and proteins in rat brain mitochondria induced by ascorbate-Fe 2+, the free radical initiator azobis(2-amidopropane)dihydrochloride (AAPH) and photosensitisation. The observed inhibitory effect was both time-and concentration-dependent. At a low concentration of 5μM, TRF can significantly inhibit oxidative damage to both lipids and proteins. The inhibitory effect of TRF seems to be mainly due to γ-tocotrienol and to a lesser extent α- and δ-tocotrienols. TRF was significantly more effective than α-tocopherol. This fraction from palm oil can be considered a natural antioxidant supplement capable of protecting the brain against oxidative damage and thereby from the ensuing adverse alterations.