An enzymatic colorimetric method for measuring naringin using 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) in the presence of peroxidase

• Published in: Analytical biochemistry Vol. 185; no. 2; pp. 335 - 338
• Main Authors: Arnao, M.B., Casas, J.L., del Río, J.A., Acosta, M., García-Cánovas, F.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.03.1990; Elsevier
• Subjects: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid); Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Benzothiazoles; Biological and medical sciences; Colorimetry - methods; Flavanones; Flavonoids - analysis; Fruit; Fundamental and applied biological sciences. Psychology; Hydrogen Peroxide; Indicators and Reagents; Oxidation-Reduction; Peroxidases; Spectrophotometry; Sulfonic Acids; Enzyme; Rutaceae; Citrus paradisi; Colorimetry; Hydrogen Peroxides; Substrate; Enzymatic method; Dicotyledones; Angiospermae; Spermatophyta; Oxidation; Peroxidase; Kinetics; Spectrophotometry; Quantitative analysis
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(90)90304-R

A sensitive colorimetric method for naringin estimation using 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as peroxidase substrate is described. The method is based on the coupling reaction of an ABTS radical cation with an oxidation product of naringin formed by peroxidase. This coupling reaction leads to the formation of a purple-colored compound with a maximum absorption at 560 nm. A molar absorption coefficient at this wavelength was calculated to be 13,286 ± 200 m −1 cm −1. The lowest amount of naringin that can be detected is 1 nmol. The application of this method to the quantification of naringin in grape-fruit tissues is presented.