Intracellular acid phosphatase and lysozyme levels in subpopulations of oyster, Crassostrea virginica, hemocytes

• Published in: Journal of invertebrate pathology Vol. 52; no. 1; pp. 163 - 167
• Main Authors: Cheng, Thomas C., Downs, James C.U.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.1988; Elsevier
• Subjects: acid phosphatase; Acid Phosphatase - metabolism; Animals; Biological and medical sciences; Blood Cells - enzymology; Crassostrea virginica; Fundamental and applied biological sciences. Psychology; granulocytes; Hemocytes - classification; Hemocytes - enzymology; hyalinocytes; Intracellular Membranes - enzymology; Invertebrates; lysozyme; Marine; Mollusca; Muramidase - metabolism; Ostreidae - enzymology; Physiology. Development; subpopulations of hemocytes; granulocytes; subpopulations of hemocytes; acid phosphatase; hyalinocytes; lysozyme; Crassostrea virginica; Enzyme; Granulocyte; Marine environment; Hyalinocyte; Blood cell; Hemocyte; Enzymatic activity; Bivalvia; Invertebrata; Mollusca; Acid phosphatase; Lysozyme
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/0022-2011(88)90116-4

Five subpopulations of Crassostrea virginica hemocytes have been separated by employing a Percoll discontinuous density gradient. As reported previously (T. C. Cheng, J. W. Huang, H. Karadogan, L. R. Renwrantz, T. P. Yoshino, J. Invertebr. Pathol. 36, 35–40, 1980), cells comprising subpopulation 2 are hyalinocytes. Quantitative determinations of the activity levels of two lysosomal hydrolases, acid phosphatase and lysozyme, have revealed that granulocytes comprising subpopulation 4 show the highest levels, followed by granulocytes comprising subpopulation 3. This and an earlier report of several differences between members of subpopulations of oyster hemocytes suggest functional differences.