Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter

• Published in: Gene Vol. 60; no. 1; pp. 65 - 74
• Main Authors: Adra, Chaker N., Boer, Poppo H., McBurney, Michael W.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 1987; Amsterdam Elsevier; New York, NY
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cloning, Molecular; Electrophoresis, Agar Gel; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Genes. Genome; HeLa Cells; introns; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; ousekeeping genes; phosphoglycerate kinase; Phosphoglycerate Kinase - genetics; Promoter Regions, Genetic; Recombinant DNA; Transfection; X-chromosome inactivation; ousekeeping genes; pgk-1; SDS; nt; EtdBr; introns; Recombinant DNA; X-chromosome inactivation; bp; PGK; kb; cDNA; phosphoglycerate kinase; Cell culture; Enzyme; Transcription promoter; Rodentia; Gene expression; Phosphoglycerate kinase; Intervening sequence; Vertebrata; Mammalia; Transfection; Gene; Mouse; X-Chromosome
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(87)90214-9

We report the cloning of the mouse pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, the potential binding site for the Sp1 transcription factor, a CCAAT sequence, but no TATA box. This promoter functions following DNA-mediated transfection into mammalian cells. The promoter of the mouse pgk-1 gene is homologous to the human pgk-1 promoter. A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the pgk-1 gene.