A biotin-avidin sandwich enzyme-linked immunosorbent assay of growth hormone in bovine plasma

Purified pituitary bovine growth hormone (bGH) has been used to develop a homologous sandwich enzyme-linked immunosorbent assay in which affinity-purified antibodies are immobilized on microtiter plates. Bovine GH bound to specific antibody is then revealed with a second anti-bGH antibody labeled wi...

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Bibliographic Details
Published inJournal of immunological methods Vol. 110; no. 1; p. 123
Main Authors Secchi, C, Biondi, P A, Berrini, A, Simonic, T, Ronchi, S
Format Journal Article
LanguageEnglish
Published Netherlands 25.05.1988
Subjects
Animals
Avidin
Biotin
Cattle - blood
Cross Reactions
Enzyme-Linked Immunosorbent Assay - methods
Growth Hormone - blood
Growth Hormone - immunology
Immune Sera
Mammals - immunology
Species Specificity
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Summary:Purified pituitary bovine growth hormone (bGH) has been used to develop a homologous sandwich enzyme-linked immunosorbent assay in which affinity-purified antibodies are immobilized on microtiter plates. Bovine GH bound to specific antibody is then revealed with a second anti-bGH antibody labeled with biotin and peroxidase-conjugated avidin. The method requires only 48 h, including the coating step, and has a sensitivity as low as 0.25 ng/ml of bGH. Statistical analyses (test of parallelism, cross-reactivity among bGH and GH of various species and bovine prolactin, recovery test, within- and between-assay variation, comparison with radioimmunoassay) confirm the high specificity and reproducibility of the method.
ISSN:0022-1759
DOI:10.1016/0022-1759(88)90091-9