Short-time cytotoxicity of mussel extracts: a new bioassay for okadaic acid detection

Okadaic acid (OA), the main toxin responsible for diarrhoeic shellfish poisoning (DSP) has high cytotoxicity for KB cell cultures (apparent after 3 hr of contact), facilitating rapid detection in contaminated mussels. We developed a method to determine the minimal active concentration (MAC) based on...

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Bibliographic Details
Published inToxicon (Oxford) Vol. 30; no. 11; p. 1419
Main Authors Amzil, Z, Pouchus, Y F, Le Boterff, J, Roussakis, C, Verbist, J F, Marcaillou-Lebaut, C, Masselin, P
Format Journal Article
LanguageEnglish
Published England 01.11.1992
Subjects
Animals
Bivalvia - metabolism
Cell Survival - drug effects
Cells, Cultured
Chromatography, High Pressure Liquid
Ethers, Cyclic - analysis
Ethers, Cyclic - toxicity
Humans
KB Cells
Liver - physiology
Okadaic Acid
Pancreas - physiology
Phosphoprotein Phosphatases - antagonists & inhibitors
Tissue Extracts - toxicity
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Summary:Okadaic acid (OA), the main toxin responsible for diarrhoeic shellfish poisoning (DSP) has high cytotoxicity for KB cell cultures (apparent after 3 hr of contact), facilitating rapid detection in contaminated mussels. We developed a method to determine the minimal active concentration (MAC) based on direct microscopic study of toxin-induced changes in cell morphology. A high correlation was found between the MAC of tested extracts and corresponding OA concentrations in mussel hepatopancreas as measured by high performance liquid chromatography. This technique is rapid and reproducible and does not require the use of living animals.
ISSN:0041-0101
DOI:10.1016/0041-0101(92)90517-9