Phorbol ester dissociates endothelin-stimulated phosphoinositide hydrolysis and arachidonic acid release in vascular smooth muscle cells

• Published in: Biochemical and biophysical research communications Vol. 160; no. 2; pp. 868 - 873
• Main Authors: Reynolds, Elwood E., Mok, Lester L.S., Kurokawa, Shingo
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 28.04.1989; Elsevier
• Subjects: Animals; Arachidonic Acid; Arachidonic Acids - metabolism; Biological and medical sciences; bovine serum albumin; BSA; Cell physiology; Cells, Cultured; DMEM/F12; Dulbecco's modified Eagle's medium/Ham's F12 (1:1); endothelin; Endothelins; Endothelium, Vascular - enzymology; Endothelium, Vascular - metabolism; Endothelium, Vascular - physiology; Fundamental and applied biological sciences. Psychology; G protein; guanine nucleotide binding regulatory protein; Hank's balanced salt solution; HBSS; Hydrolysis; inositol phosphate; Inositol Phosphates - biosynthesis; Molecular and cellular biology; Muscle, Smooth, Vascular - enzymology; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - physiology; Peptides - metabolism; Peptides - physiology; Pertussis Toxin; phorbol 12-myristate 13-acetate; Phosphatidylinositols - metabolism; phosphoinositides; phospholipase A 2; phospholipase C; Phospholipases A - metabolism; PLA 2; PLC; PMA; rabbit renal artery vascular smooth muscle cells; Rabbits; Receptors, Adrenergic, alpha - drug effects; Responses to growth factors, tumor promotors, other factors; RRA-VSM; Tetradecanoylphorbol Acetate - pharmacology; Type C Phospholipases - metabolism; Virulence Factors, Bordetella; AA; BSA; RRA-VSM; PT; PMA; PLC; IP; DMEM/F12; HBSS; PLA 2; G protein; Performance evaluation; Hormone; Cell culture; Radiolabelling; Peptides; Rodentia; Rabbit; Smooth muscle; Stimulation; Activation; Lagomorpha; Arachidonic acid; Metabolism; Fatty acids; In vitro; Kidney; Tissue; Vertebrata; Regulation(control); Mammalia
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(89)92515-1

Endothelin-stimulated { 3H}-inositol phosphate formation and { 3H}-arachidonic acid release were measured in cultured vascular smooth muscle cells from rabbit renal artery. Both responses were partially inhibited by pretreatment with pertussis toxin, indicating the involvement of pertussis toxin-sensitive guanine nucleotide binding regulatory proteins in the coupling processes. Pretreatment with the phorbol ester PMA inhibited endothelin-stimulated { 3H}-inositol phosphate formation, but potentiated endothelin-stimulated { 3H}-arachidonic acid release, suggesting that these two coupling processes occur in a parallel and independent manner in vascular smooth muscle cells.