Superoxide production induced by short-term exposure of barley roots to cadmium, auxin, alloxan and sodium dodecyl sulfate

• Published in: Plant cell reports Vol. 31; no. 12; pp. 2189 - 2197
• Main Authors: LIPTAKOVA, L'ubica, BOCOVA, Beáta, HUTTOVA, Jana, MISTRIK, Igor, TAMAS, Ladislav
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.12.2012; Springer; Springer Nature B.V
• Subjects: alloxan; Alloxan - pharmacology; Barley; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cadmium; Cadmium - pharmacology; Cell Biology; Cell Death; Enzyme Activation; Fundamental and applied biological sciences. Psychology; growth retardation; Hordeum - drug effects; Hordeum - growth & development; Hordeum - metabolism; indole acetic acid; Indoleacetic Acids - pharmacology; Life Sciences; NADPH Oxidases - metabolism; Original Paper; Plant Biochemistry; Plant Cells - drug effects; Plant Cells - metabolism; Plant Epidermis - drug effects; Plant Epidermis - metabolism; Plant growth; Plant Roots - drug effects; Plant Roots - growth & development; Plant Roots - metabolism; Plant Sciences; rhizosphere; root growth; root tips; sodium dodecyl sulfate; Sodium Dodecyl Sulfate - pharmacology; Stress, Physiological; Superoxides - metabolism; Time Factors; Cadmium; Root growth inhibition; Superoxide; Cell death; Root swelling; Short term; Growth inhibitor; Monocotyledones; Vegetals; Auxin; Swelling; Hordeum vulgare; Root; Growth; Lauryl sulfate; Anionic surfactant; Hyperoxides; Sodium; Gramineae; Angiospermae; Production; Spermatophyta; Inhibition
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-012-1329-6

KEY MESSAGE : Abiotic stress-induced superoxide generation depending on its localization, level, duration and presumably also on the action of other signals may lead to different stress responses. The purpose of this study was to analyze the alterations in superoxide generation and morphogenesis following short-term Cd, IAA and alloxan treatments, during stress and recovery period in barley root tips. At low Cd concentration the transient accumulation of superoxide in the epidermal cells was accompanied by root growth inhibition and radial expansion of cortical cells in the elongation zone of root tips. These morphological changes were very similar to the externally applied IAA-induced responses. However, the role of superoxide generated in the epidermal cells by low concentration of Cd and IAA is probably alone not sufficient for the induction of these processes. SDS as an activator of NOX activity caused a strong accumulation of superoxide in the epidermal cells along the whole root apex but without any changes in root morphology and growth. On the other hand, higher Cd concentrations as well as alloxan stimulated the generation of superoxide in the cortical tissue of the elongation zone of root tip, which was accompanied by the induction of cell death. Our results suggest that enhanced superoxide generation, depending on its localization, level, duration and presumably also on the action of other signals, may lead to altered root morphology (15 μM Cd or IAA), root growth inhibition (alloxan), transient root growth cessation (30 μM Cd) or to the death of cells/root at higher (60 μM) Cd concentrations.