Internucleosomal DNA fragmentation in gerbil hippocampus following forebrain ischemia

• Published in: Neuroscience letters Vol. 171; no. 1; pp. 179 - 182
• Main Authors: Sei, Yoshitatsu, Von Lubitz, Dag K.J.E., Basile, Anthony S., Borner, Markus M., Lin, Rick C.-S., Skolnick, Phil, Fossom, Linda H.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 25.04.1994; Elsevier
• Subjects: Animals; Apoptosis; Apoptosis - physiology; Biological and medical sciences; Brain Ischemia - metabolism; Brain Ischemia - pathology; Cerebrovascular Circulation - physiology; Cytidine Triphosphate - metabolism; DNA - biosynthesis; DNA - metabolism; DNA fragmentation; Female; Gerbillinae; Hippocampus; Hippocampus - metabolism; Hippocampus - pathology; In Situ Hybridization; Ischemia; Medical sciences; Meriones unguiculatus; Neurology; Nucleosomes - metabolism; Programmed cell death; Prosencephalon - blood supply; Vascular diseases and vascular malformations of the nervous system; Programmed cell death; Ischemia; Hippocampus; DNA fragmentation; Apoptosis; Nervous system diseases; Rodentia; Fragmentation; Pathology; Vertebrata; Mammalia; Cell death; Animal; DNA; Central nervous system disease; Gerbil; Cerebrovascular disease; Brain (vertebrata)
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/0304-3940(94)90634-3

Internucleosomal DNA fragmentation, the characteristic feature of programmed cell death, was demonstrated in gerbil hippocampus following 10 min of forebrain ischemia. Quantitative analysis revealed the presence of DNA fragments as early as 12 h after ischemia, reaching a maximum at 48 h. Measurable DNA fragmentation was still present in 3 3 subjects 96 h after the ischemic insult. In situ staining of hippocampus demonstrated pronounced DNA fragmentation that was localized in the CA1 region. The localization of fragmented DNA to the CA1 is consistent with the vulnerability of this layer to ischemic insult, and indicates that DNA fragmentation may be associated with the delayed loss of CA1 neurons in this model of forebrain ischemia.