Transcription of the human adenovirus E1a gene in Saccharomyces cerevisiae

• Published in: Gene Vol. 33; no. 2; pp. 159 - 168
• Main Authors: Hiroshi, Handa, Kiyohisa, Mizumoto, Kinichiro, Oda, Takashi, Okamoto, Toshio, Fukasawa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 1985
• Subjects: Adenoviridae; Adenoviruses, Human - genetics; E. coli shuttle vector; gene expression; Gene Expression Regulation; Genes, Viral; mRNA; Promoter Regions, Genetic; Recombinant DNA; RNA Caps; RNA Processing, Post-Transcriptional; RNA Splicing; RNA, Viral - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Transcription, Genetic; viral gene; yeast; yeasts; SD; Ad5; SDS; E1a; viral gene; E. coli shuttle vector; kb; mRNA; Recombinant DNA; indicates plasmid-carrier state; yeast; bp
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(85)90090-3

The early region 1a (E1a) and its flanking sequences of human adenovirus type 5 (Ad5) have been cloned in the yeast- Escherichia coli shuttle vector YEp13 and transferred into the yeast Saccharomyces cerevisiae. The E1a-specific RNAs were produced in the transformed yeast cells. The 5' ends of these transcripts were capped but were lacking 10 to 45 nucleotides from the 5' end of the proper E1a mRNA. These transcripts terminated approx. 1000 nucleotides downstream from the proper 3' end. No splicing of the E1a-specific RNA could be detected in the yeast cells.