An RFLP map of the Plasmodium falciparum genome, recombination rates and favored linkage groups in a genetic cross

• Published in: Molecular and biochemical parasitology Vol. 51; no. 2; pp. 313 - 320
• Main Authors: Walker-Jonah, Annie, Dolan, Stephen A., Gwadz, Robert W., Panton, Lindsey J., Wellems, Thomas E.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.04.1992; Elsevier Science
• Subjects: Animals; Anopheles - parasitology; Biological and medical sciences; Chromosome mapping; Crosses, Genetic; Fundamental and applied biological sciences. Psychology; Genes. Genome; Genetic Linkage; Genetic Markers; Genetic recombination; Linkage analysis; Malaria; Molecular and cellular biology; Molecular genetics; Plasmodium falciparum; Plasmodium falciparum - genetics; Polymorphism, Restriction Fragment Length; Recombination, Genetic; Restriction Mapping; Tropical medicine; Plasmodium falciparum; Chromosome mapping; Malaria; Genetic recombination; Linkage analysis; Protozoa; Recombination; Restriction fragment length polymorphism; Linkage group; Pathogen; Genome; Sporozoa
• ISSN: 0166-6851; 1872-9428
• DOI: 10.1016/0166-6851(92)90081-T

We report a genetic linkage map of the Plasmodium falciparum genome, using the inheritance patterns of nearly 90 RFLP markers in a genetic cross. Markers were assigned to polymorphic loci on all 14 nuclear chromosomes. Genetic recombination between parental markers was detected in each of the progeny, indicating that progeny from cross-fertilization events were favored over progeny from self-fertilization of either parent alone. Inheritance patterns among the markers suggested that certain parental linkage groups on chromosomes 2,3,12 and 13 were favored in the cross. Recombination frequencies on five chromosomes indicated an approximate map unit size of 15–30 kb per centiMorgan for P. falciparum.