Simultaneous HPLC analysis of the hypnotic benzodiazepines nitrazepam, estazolam, flunitrazepam, and triazolam in plasma

A new analytical methodology was developed for simultaneous high-performance liquid chromatographic quantitation of nitrazepam, estazolam, flunitrazepam, and triazolam in plasma. After addition of the internal standard nor-prazepam, biological samples and calibration standards were extracted at basi...

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Bibliographic Details
Published inJournal of analytical toxicology Vol. 15; no. 6; p. 319
Main Authors Boukhabza, A, Lugnier, A A, Kintz, P, Mangin, P
Format Journal Article
LanguageEnglish
Published England 01.11.1991
Subjects
Chromatography, High Pressure Liquid
Estazolam - blood
Flunitrazepam - blood
Humans
Nitrazepam - blood
Triazolam - blood
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Summary:A new analytical methodology was developed for simultaneous high-performance liquid chromatographic quantitation of nitrazepam, estazolam, flunitrazepam, and triazolam in plasma. After addition of the internal standard nor-prazepam, biological samples and calibration standards were extracted at basic pH into diethyl ether-methylene chloride (2:1, v/v). The reconstituted extract was separated on a reversed-phase Nova Pak C18 column with acidic buffer (6mM)-acetonitrile-methanol (64:23:13, v/v) as mobile phase. The detection was performed at 242 nm. Within-run and day-to-day precision values were about 2-8%. The method is thus sensitive and reproducible for use in clinical and experimental toxicokinetic studies.
ISSN:0146-4760
DOI:10.1093/jat/15.6.319