Variations in lethal and sublethal effects of cypermethrin among aquatic stages and species of anuran amphibians

• Published in: Environmental toxicology and chemistry Vol. 32; no. 12; pp. 2855 - 2860
• Main Authors: Biga, Lindsay M., Blaustein, Andrew R.
• Format: Journal Article
• Language: English
• Published: Pensacola, FL Blackwell Publishing Ltd 01.12.2013; SETAC
• Subjects: Amphibian; Amphibians; Animal, plant and microbial ecology; Animals; Anura - embryology; Anura - growth & development; Applied ecology; Aquatic habitats; Biological and medical sciences; Cypermethrin; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Embryo; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - physiology; Embryology: invertebrates and vertebrates. Teratology; Embryos; Exposure; Frogs; Fundamental and applied biological sciences. Psychology; Habitats; Hatching; Larva; Larva - drug effects; Larva - growth & development; Larvae; Mathematical models; Morphology; Mortality; Organisms; Pesticides; Pesticides - toxicity; Pseudacris regilla; Pyrethrins - toxicity; Pyrethroid; Rana aurora; Rana cascadae; Ranidae - embryology; Ranidae - growth & development; Reptilia. Amphibia; Risk; Risk assessment; Species Specificity; Sublethal effects; Teratology. Teratogens; Time Factors; Toxic effect; Water Pollutants, Chemical - toxicity; Xenobiotics; Pyrethroid; Embryo; Insecticide; Toxicity; Pesticides; Amphibia; Pyrethroids; Salientia; Survival; Toxic effect; Lethal dose; Aquatic environment; Pollutant; Vertebrata; Sublethal dose; Larva; Cypermethrin; Teratogen; Amphibian
• ISSN: 0730-7268; 1552-8618; 1552-8618
• DOI: 10.1002/etc.2379

Despite the use of model species to predict the effects of chemicals in the environment, unpredicted variation in levels of risk to organisms from xenobiotics can be observed. Physiological and morphological differences between species and life stages may lead to differences in sensitivity, while seasonal and spatial variation in pesticide concentrations may affect the level of risk faced by organisms in the environment. Because anurans breed in aquatic habitats subject to contamination by runoff and spraying, they are particularly vulnerable to pesticides. In the present study, embryos, newly hatched larvae, and larvae with limb buds of 3 anuran amphibian species—Pseudacris regilla, Rana cascadae, and Rana aurora—were exposed for 48 h to either 0.5 µg/L or 5.0 µg/L cypermethrin under laboratory conditions. The authors monitored hatching success, larval survival, and measured growth. Additionally, they assayed avoidance behavior 2 wk after exposure or 2 wk after hatching for individuals exposed as embryos. Hatching and survival were not affected in animals of any species exposed as embryos. After exposure as embryos and as newly hatched larvae, however, P. regilla displayed behavioral abnormalities in response to prodding. Cypermethrin increased mortality in P. regilla exposed in both larval stages. Cypermethrin also increased mortality in larval R. cascadae when exposed at the early stage. These results indicate variation in sensitivity to environmentally relevant concentrations of cypermethrin among anuran species and life stages. Environ Toxicol Chem 2013;32:2855–2860. © 2013 SETAC