Structure of the gas vesicle protein GvpF from the cyanobacterium Microcystis aeruginosa
Gas vesicles are gas‐filled proteinaceous organelles that provide buoyancy for bacteria and archaea. A gene cluster that is highly conserved in various species encodes about 8–14 proteins (Gvp proteins) that are involved in the formation of gas vesicles. Here, the first crystal structure of the gas...
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Published in | Acta crystallographica. Section D, Biological crystallography. Vol. 70; no. 11; pp. 3013 - 3022 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
5 Abbey Square, Chester, Cheshire CH1 2HU, England
International Union of Crystallography
01.11.2014
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Gas vesicles are gas‐filled proteinaceous organelles that provide buoyancy for bacteria and archaea. A gene cluster that is highly conserved in various species encodes about 8–14 proteins (Gvp proteins) that are involved in the formation of gas vesicles. Here, the first crystal structure of the gas vesicle protein GvpF from Microcystis aeruginosa PCC 7806 is reported at 2.7 Å resolution. GvpF is composed of two structurally distinct domains (the N‐domain and C‐domain), both of which display an α+β class overall structure. The N‐domain adopts a novel fold, whereas the C‐domain has a modified ferredoxin fold with an apparent variation owing to an extension region consisting of three sequential helices. The two domains pack against each other via interactions with a C‐terminal tail that is conserved among cyanobacteria. Taken together, it is concluded that the overall architecture of GvpF presents a novel fold. Moreover, it is shown that GvpF is most likely to be a structural protein that is localized at the gas‐facing surface of the gas vesicle by immunoblotting and immunogold labelling‐based tomography. |
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Bibliography: | istex:BDA3E103076724CA1A4D6CB5D639E547C7DC0890 ark:/67375/WNG-184RGQW6-6 ArticleID:AYD2QH5017 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1399-0047 0907-4449 1399-0047 |
DOI: | 10.1107/S1399004714021312 |