Cytological investigation of R1 and R2-generations of spontaneously and colchicine induced diploid anther derived wheat plants

• Published in: CYTOLOGIA Vol. 63; no. 3; pp. 267 - 278
• Main Authors: Redha, A, Schmid, J.E, Buter, B, Stamp, P, Attia, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Mendel Society, International Society of Cytology 1998; Japan Science and Technology Agency
• Subjects: Anther culture; Colchicine; Cytology; Doubled haploid wheat; Gametoclonal variation; Meiotic behaviour; Triticum aestivum
• ISSN: 0011-4545; 1348-7019
• DOI: 10.1508/cytologia.63.267

Evaluation of cytological stability has been made for R1 and R2 generations of anther derived doubled haploid wheat plants developed either spontaneously or in response to the addition of 100 mg/l or 1000 mg/l colchicine to induction media for a duration of 1, 3 and 5 days. Determined abnormalities in meiotic behaviour in chromosomal structure and number offered a confirmation for the role of culture conditions in generating gametoclonal variations in regenerated plants. Frequent failure of pairing, occurrence of chromosomal translocations and breaks in addition to aneuploidy were detectable in regenerated DH-plants but not in their parental anther donor control. Interpretations of origin and possible effects of detected types of gametoclonal variations in relation to potential value of anther culture technique has been proposed and discussed. Colchicine treatment had not affected the degree of cytological instability, since no significant differences were found between spontaneously arisen doubled haploids and those developed after treatments by two colchicine concentrations and three durations. The importance of closely monitoring regenerants of anther culture through a cytological evaluation for a safe and effective application in breeding programs is stressed.