DIAMINE-THIOCARBOHYDRAZIDE-SILVER PROTEINATE-PHYSICAL DEVELOPMENT METHODS FOR THE HISTOCHEMICAL DETECTION OF ACIDIC GLYCOCONJUGATES IN ELECTRON MICROSCOPY

A couple of new sensitive methods have been established for the histochemical detection of acidic glycoconjugates in electron microscopy. These are combined staining methods consisting of a high or low iron diamine preembedding and thiocarbohydrazide silver proteinate postembedding sequence (HID or...

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Bibliographic Details
Published inACTA HISTOCHEMICA ET CYTOCHEMICA Vol. 23; no. 3; pp. 281 - 294
Main Author KITAMURA, HIROKAZU
Format Journal Article
LanguageEnglish
Published Kyoto JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 01.01.1990
Japan Society of Histochemistry and Cytochemistry
Japan Science and Technology Agency
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Proteins
Staining
Electron microscopy
Glycoconjugate
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Summary:A couple of new sensitive methods have been established for the histochemical detection of acidic glycoconjugates in electron microscopy. These are combined staining methods consisting of a high or low iron diamine preembedding and thiocarbohydrazide silver proteinate postembedding sequence (HID or LID-TCH-SP) followed by a physical development (PD) procedure. The HID or LID-TCH-SP-PD methods have been applied to a series of acidic glycoconjugate-containing ultrastructures from different organs of the rat and mouse such as the aorta, kidney, pancreas and spleen, in comparision with the HID or LID-TCH-SP techniques. The new methods are signigicantly more sensitive than the HID or LID-TCH-SP technique. Their specificity is sufficient.
ISSN:0044-5991
1347-5800
DOI:10.1267/ahc.23.281