UGT1A128 detection using high-resolution agarose gel electrophoresis

A new UGT1A1*28 detection method combining PCR and high-resolution agarose gel electrophoresis was developed. The viability of this method was demonstrated on 15 healthy adult volunteers. Subjects included 13 wild type homozygotes (86.7 %), 2 heterozygotes (13.3 %), and no mutant type homozygotes (0...

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Published inHeliyon Vol. 10; no. 9; p. e30465
Main Authors Tsuchida, Shirou, Hirayama, Takaaki, Nunose, Hayato, Suzuki, Hinako, Hakota, Ryo, Shindo, Tsugumi, Nakagawa, Koji
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 15.05.2024
Elsevier
Subjects
Agarose gel electrophoresis
Genotyping
PCR
UGT1A128
UGT1A128
Genotyping
Agarose gel electrophoresis
PCR
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Summary:A new UGT1A1*28 detection method combining PCR and high-resolution agarose gel electrophoresis was developed. The viability of this method was demonstrated on 15 healthy adult volunteers. Subjects included 13 wild type homozygotes (86.7 %), 2 heterozygotes (13.3 %), and no mutant type homozygotes (0 %). The new UGT1A1*28 detection method results were fully consistent with DNA sequencing. PCR and agarose gel electrophoresis are common techniques with high-resolution agarose gels available commercially. These results support the clinical viability of this method potentially reducing UGT1A1*28 diagnosis complexity and cost.
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ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2024.e30465