Fast and interference-free determination of glyphosate and glufosinate residues through electrophoresis in disposable microfluidic chips

• Published in: Journal of Chromatography A Vol. 1281; pp. 148 - 154
• Main Authors: Wei, Xuan, Gao, Xiaotong, Zhao, Lei, Peng, Xianglu, Zhou, Lei, Wang, Jing, Pu, Qiaosheng
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.03.2013; Elsevier
• Subjects: Aminobutyrates - analysis; Aminobutyrates - chemistry; Analysis methods; Analytical chemistry; Applied sciences; Biological and medical sciences; Borates - chemistry; borax; Brassica - chemistry; cellulose; Chemistry; Chromatographic methods and physical methods associated with chromatography; chromatography; composite polymers; cost analysis; Cyclic olefin copolymer; derivatization; electrophoresis; Electrophoresis, Microchip - instrumentation; Electrophoresis, Microchip - methods; Exact sciences and technology; fluorescence; Food industries; food safety; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Glufosinate; Glycine - analogs & derivatives; Glycine - analysis; Glycine - chemistry; Glycine max - chemistry; Glyphosate; herbicide residues; Herbicides - analysis; Herbicides - chemistry; Hydrogen-Ion Concentration; Laser induced fluorescence; Limit of Detection; Microchip electrophoresis; Natural water pollution; olefin; Other chromatographic methods; Pesticide Residues - analysis; Pesticide Residues - chemistry; Pollution; Regression Analysis; Reproducibility of Results; screening; Water treatment and pollution; Microchip electrophoresis; Glyphosate; Cyclic olefin copolymer; Glufosinate; Laser induced fluorescence; Chemical analysis; Vegetables; Systemic; Soybean; Derivatization; Phosphorus Organic compounds; Microequipment; Quantitative analysis; Disposable; Validation; Fluid mechanics; Fluorescence detector; Capillary electrophoresis; Pesticides; Organic phosphonate; System on a chip; Contamination; Herbicide; α-Aminoacid; Broccoli; Cycloalkene copolymer; Fluorescent labelling; Residue; Aminoacid; Surface water; Water pollution; Miniaturization; River water; Microfluidics
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2013.01.039

A fast and interference-free method based on microchip electrophoresis with laser induced fluorescence detection was established for the determination of trace amount of glyphosate and glufosinate residues in agricultural products. [Display omitted] ► Microchip electrophoresis was adopted for analysis of glyphosate and glufosinate. ► Easy-to-make low-cost plastic microchips and hardwares were utilized. ► Anodic separation was used to get rid of potential interferences. ► The method was fast, sensitive and excellent in interference tolerance. ► The method was applied in the analysis of real samples with complicated matrices. With an increasing concern on food safety, fast screening of residues of widespread herbicides becomes necessary. Herein we report a microchip electrophoresis system with laser induced fluorescence (LIF) detection for rapid and sensitive analysis of glyphosate (GLYP) and glufosinate (GLUF) residues. Disposable cyclic olefin copolymer microchips and a low-cost LIF detector were employed to minimize the cost of the analysis. Systematic optimization of experimental conditions was performed to achieve highly efficient analysis. Under the selected condition, GLYP and GLUF were efficiently resolved from sample matrices with a buffer containing 10mmol/L borax and 2.0% (m/v) hydroxypropyl cellulose at pH 9.0. The number of theoretical plates of 1.0×106m–1 was attained for both analytes. Derivatization at lower concentrations (<10μg/L) was also examined, successful detection of 0.34μg/L GLYP and 0.18μg/L GLUF was confirmed. The system was applied for the determination of both analytes in real samples without any preconcentration involved. Recoveries of GLYP and GLUF spiked in these samples were 84.0–101.0% and 90.0–103.0%, respectively.