Ultrasound-assisted surfactant-enhanced emulsification microextraction for the determination of carbamates in wines by ultra-high performance liquid chromatography–tandem mass spectrometry

• Published in: Journal of Chromatography A Vol. 1315; pp. 1 - 7
• Main Authors: Moreno-González, David, Huertas-Pérez, José F., García-Campaña, Ana M., Bosque-Sendra, Juan M., Gámiz-Gracia, Laura
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 08.11.2013; Elsevier
• Subjects: Biological and medical sciences; Carbamates; Carbamates - analysis; Chemical Fractionation - methods; Chromatography; Chromatography, High Pressure Liquid - methods; Cleaning; compliance; Emulsification; emulsifying; Emulsions; Extraction; Fermented food industries; Food industries; formic acid; fortified wines; Fundamental and applied biological sciences. Psychology; liquid chromatography; Liquids; Mass spectrometry; methanol; Methyl alcohol; microextraction; monitoring; multiresidue analysis; Reproducibility of Results; Sensitivity and Specificity; solvents; Sonication; tandem mass spectrometry; Tandem Mass Spectrometry - methods; UASEME; UHPLC–MS/MS; Wine; Wine - analysis; Wines; Wines and vinegars; UASEME; Wine; Carbamates; UHPLC–MS/MS; Ultra performance liquid chromatography; Tandem mass spectrometry; Chemical analysis; Insecticide; Emulsification; Surfactant; Solvent extraction; Multiresidue analysis; Organic carbamate; Electrospray; Chemical enrichment; Ultrasound assisted extraction; Sample preparation; Ultrasound; Quantitative analysis; Validation; Coupled method; Pesticides; Contamination; Operating conditions; UHPLC―MS/MS; Reversed phase chromatography; Residue; Liquid liquid microextraction
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2013.09.028

•An UHPLC–MS/MS method is proposed for determination of 25 carbamates in different wines.•In order to obtain clean extracts, a new sample treatment based on UASEME has been developed.•LOQs are below maximum residue levels allowed by EU in the case of grape wines.•The proposed method could be a good alternative for multi-carbamate determination in wine samples. A new sensitive multiresidue method based on ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) has been developed for the detection, confirmation and quantification of twenty five carbamates in wine samples. The separation was achieved in 5.5min, using a Zorbax Eclipse plus RRHD C18 column (50mm×2.1mm, 1.8μm), with a mobile phase of water and methanol, both of them with 0.01% formic acid. The analytes were detected in positive mode with multiple reaction monitoring mode. Ultrasound-assisted surfactant-enhanced emulsification microextraction (UASEME), using a low-density extraction solvent has been optimized for the satisfactory extraction of carbamates and clean-up of extracts. The matrix effect was studied, showing that the proposed procedure provides very clean extracts. Under optimum conditions, recoveries for fortified wine samples ranged from 74 to 102%, with relative standard deviations lower than 6%. Limits of quantification ranged from 0.15 to 0.92μgl−1, showing the high sensitivity of this fast and simple method and its compliance with current requirements.