Vaccination with TCL plus MHSP65 induces anti-lung cancer immunity in mice

• Published in: Cancer Immunology, Immunotherapy Vol. 59; no. 6; pp. 899 - 908
• Main Authors: Dong, Bohan, Sun, Luguo, Wu, Xiuli, Zhang, Peiyin, Wang, Li, Wei, Hongfei, Zhou, Lei, Hu, Xiaoping, Yu, Yongli, Hua, Shucheng, Wang, Liying
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.06.2010; Springer-Verlag; Springer Nature B.V
• Subjects: Adjuvants, Immunologic - administration & dosage; Animals; Antigens, Neoplasm - administration & dosage; Antigens, Neoplasm - immunology; Bacterial Proteins - administration & dosage; Bacterial Proteins - immunology; Cancer Research; Cancer Vaccines; Carcinoma, Lewis Lung - immunology; Carcinoma, Lewis Lung - metabolism; Carcinoma, Lewis Lung - pathology; Carcinoma, Lewis Lung - therapy; Cell Extracts - administration & dosage; Cell Extracts - immunology; Cell Growth Processes - drug effects; Cell Growth Processes - immunology; Chaperonin 60 - administration & dosage; Chaperonin 60 - immunology; Female; Immunity, Cellular - drug effects; Immunization; Immunology; Lung cancer; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Mycobacterium; Neoplasm Transplantation; Oncology; Original; Original Article; Tumor Burden - drug effects; Tumor Burden - immunology; Tumor cell lysate; Lung cancer; Heat shock protein; Anti-tumor immunity
• ISSN: 0340-7004; 1432-0851
• DOI: 10.1007/s00262-010-0816-2

To develop effective anti-lung cancer vaccines, we directly mixed mycobacterial heat shock protein 65 (MHSP65) and tumor cell lysate (TCL) from Lewis lung cancer cells in vitro and tested its efficacy on stimulating anti-tumor immunity. Our results showed that MHSP65-TCL immunization significantly inhibited the growth of lung cancer in mice and prolonged the survival of lung cancer bearing mice. In vivo and in vitro data suggest that MHSP65-TCL could induce specific CTL responses and non-specific immunity, both of which could contribute to the tumor inhibition. Thus, this report provides an easy approach to prepare an efficient TCL based tumor vaccine.